Iranian J Pharmacol Ther. 2017 (June);15:1-7. This paper is available online at: http://ijpt.iums.ac.ir Experimental study of cerium oxide nanoparticles (CeNP) against malathion induced lung oxidative toxic stress in rats Maziar Ganji 1 , Jamshid Karimi 2 , Seyed Abdolhakim Hosseini 3 , Heresh Moridi 2,3 , Asieh Hosseini 4 , Davoud Ahmadimoghaddam 5 , Akram Ranjbar 5 * 1 Student Research Center, Hamadan University of Medical Sciences, Hamadan, Iran, & Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Sciences, Tehran, Iran 2 Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran 3 Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 4 Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran 5 Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran Please cite this article as: Ganji M, Karimi J, Hosseini SA, Moridi H, Hosseini A, Ahmadimoghaddam D, Ranjbar A. Experimental study of cerium oxide nanoparticles (CeNP) against malathion induced lung oxidative toxic stress in rats. Iranian J Pharmacol Ther. 2017 (July);15: 1-7. ABSTRACT Regardless of toxicity of nanoparticles, cerium oxide nanoparticles (CeNPs) are emerging as a multi-functional agent for biomedical purposes. On the other hand, Organophosphorus pesticides, like malathion, are inevitably found in the environment. The common involving pathway CeNPs and malathion share is oxidative stress. Therefore, we conducted this study to find the possible neutralizing or synergistic effects of CeNPs on oxidative stress responses in malathion-induced toxicity by intraperitoneal (IP) injection. In this experimental study, 40 Wistar male rats with the weight range of 200-250 g were randomly selected and divided into eight groups. Group1 (control, normal saline), group2 (100 mg/kg/day malathion /IP), group3 (15 mg/kg/day CeNPs/IP), group4 (30 mg/kg/day CeNPs /IP), group5 (60 mg/kg/day CeNPs /IP), group6 (100 mg/kg/day malathion+15 mg/kg/day CeNPs /IP), group7 (100 mg/kg/day malathion+30 mg/kg/day CeNPs /IP) and group8 (100mg/kg/day malathion+60 mg/kg/day CeNPs /IP). After 4 weeks of treatment, the levels of lipid peroxidation (LPO), total antioxidant capacity (TAC), total thiol molecules (TTM) and activity of catalase (CAT) in lung tissue were measured. All data were analyzed by SPSS V16 and One way ANOVA with Tukey post hoc test. The results demonstrated that CeNPs caused significant increases in LPO and TAC, in a dose-dependent-manner. For TTM level, none of the groups presented any significant change compared to control. Significantly decreased levels of CAT, also, were seen in all treatment groups. Surprisingly, all animals of group 8 died. Worth of noting, groups receiving combined CeNPs and malathion showed severe responses for these parameters. These results discovered that CeNPs induces oxidative stress parameters and ROS production, especially combined with malathion in lung tissue. Groups receiving both CeNPs and malathion displayed synergistic toxic properties. LPO, TAC and CAT seem to be better parameters for measuring CeNPs-induced responses. Further investigations are required to shed light on clear mechanisms involved. Conflicts of Interest: Declared None Funding: None Keywords Cerium oxide nanoparticle, Lung, Oxidative Stress, Rat, Malathion Corresponding to: Akram Ranjbar, Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran Email: akranjbar2015@gmail.com Received: 6 May 2016 Revised: 2 July 2016, Accepted: 25 July 2016 INTRODUCTION Organophosphorus compounds are known to be one of the most widely utilized pesticides all over the world. Residual amounts of these pesticides can be detected in the Original Article IRANIAN JOURNAL OF PHARMACOLOGY & THERAPEUTICS Copyright © 2017 by Iran University of Medical Sciences Iranian J Pharmacol Ther. 2017 (July);15:1-7. Downloaded from ijpt.iums.ac.ir at 10:57 IRST on Saturday November 27th 2021