Journal of Biosciences and Medicines, 2021, 9, 26-42 https://www.scirp.org/journal/jbm ISSN Online: 2327-509X ISSN Print: 2327-5081 DOI: 10.4236/jbm.2021.96003 Jun. 4, 2021 26 Journal of Biosciences and Medicines Towards on the Mediators in Collagen Replacement: Effect of the Active Molecules into a Polymeric Biomaterial Anderson Ricardo Ingracio 1 , Natália Fontana Nicoletti 2 , Fernanda Trindade Gonzalez Dias 3 , Manuela Peletti-Figueiró 2* , Otávio Bianchi 1,3 , Asdrubal Falavigna 1,2 1 Health Sciences Graduate Program, Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil 2 Cell Therapy Laboratory (LATEC), Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil 3 Materials Science Graduate Program (PGMAT), Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil Abstract Modulating healing factors could avoid or minimize some possible patholog- ical processes in collagen deposition. The present study was aimed to eva- luated the role of active biomolecules such as PDGF-BB and PRP loaded or not into polymeric biomaterial to seek potential mediators in types I and III collagen deposition and epithelization. The healing phases were investigated by using an in vivo full-thickness wound rat model. At zero, 3 rd , 7 th and 14 th days after the experimental model, the size of the wound areas was photo- graphed. The nanofibrous materials were biocompatible and did not cause any local adverse reaction and/or inflammation. On day 14 the wounds had healed almost 100% with better signs of healing, however there was no ob- vious difference in the wound contraction rates. At the end of 14 days, sam- ples from the center of the lesion were collected when histological features and immunopositivity for collagen I and III expressions were assessed. There was no significant difference in the epithelization among the groups. Wounds treated with PRP and with PA-6/SOMA plus PDGF-BB had significantly lower amounts of type III collagen. The amounts of type I collagen did not have a statistically different deposition among the experimental groups. The association of PDGF-BB with PA-6/SOMA emerges as an alternative for top- ical application to unfavorable anatomical sites, suggesting that these associa- tions may have a positive modulation on the process of accelerated healing remodeling. Keywords Collagen, Fibrosis, Platelet-Rich Plasma, Polyamide-6 and Biomaterial How to cite this paper: Ingracio, A.R., Nicoletti, N.F., Dias, F.T.G., Peletti-Figueiró, M., Bianchi, O. and Falavigna, A. (2021) Towards on the Mediators in Collagen Replacement: Effect of the Active Mole- cules into a Polymeric Biomaterial. Journal of Biosciences and Medicines, 9, 26-42. https://doi.org/10.4236/jbm.2021.96003 Received: April 24, 2021 Accepted: June 1, 2021 Published: June 4, 2021 Copyright © 2021 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access