514 https://www.id-press.eu/mjms/index Scientifc Foundation SPIROSKI, Skopje, Republic of Macedonia Open Access Macedonian Journal of Medical Sciences. 2020 Apr 20; 8(B):514-518. https://doi.org/10.3889/oamjms.2020.4180 eISSN: 1857-9655 Category: B - Clinical Sciences Section: Gynecology and Obstetrics The Level Assessment of Extracellular Nucleic Acids in the Blood of Pregnant Women with Chronic Hypertension with Superimposed Preeclampsia Dinara Omertayeva 1 *, Larissa Muravlyova 1 , Olga Ponomaryova 1 , Vilen Molotov-Luchanskyi 2 , Ryszhan Bakirova 2 , Dmitryi Klyuev 1 , Miras Mugazov 3 1 Department of Fundamental Medicine, National Join-stock Company Karaganda Medical University, Kazakhstan; 2 Department of Therapy, National Join-stock Company Karaganda Medical University, Kazakhstan; 3 Department of Surgical Diseases, National Join-stock Company Karaganda Medical University, Kazakhstan Abstract BACKGROUND: Preeclampsia, chronic arterial hypertension is one of the causes of maternal and perinatal morbidity and mortality. AIM: The aim of our study was to assess the level of extracellular nucleic acids in red blood cells and blood plasma in pregnant women with severe preeclampsia, severe chronic hypertension, and pregnant women with chronic hypertension with superimposed preeclampsia. METHODS: A total of 107 pregnant women were examined who were hospitalized at the Regional Clinical Hospital and at the Regional Perinatal Center in Karaganda and 29 healthy non-pregnant women. Pregnant women were divided into four groups: 1 group – 32 pregnant with severe chronic hypertension, 2 group – 48 pregnant women with severe preeclampsia, 3 group – 35 women entered the group with chronic hypertension with superimposed preeclampsia, and group control – 29 healthy non-pregnant women of child bearing age (4 th group – control). RESULTS: The content of ecNA in the erythrocytes of pregnant women with chronic hypertension with superimposed preeclampsia sharply increases relative to the control and comparison groups; in pregnant women with severe hypertension and severe preeclampsia, the level of ecNA is moderately higher relative to the control, but lower than in the main group. CONCLUSION: Our data indicate signifcant changes in the level of ecNA in pregnant women with various types of hypertension and require further research, but now, changes in the concentration of ecNA can be considered as one of the links in the pathogenesis of preeclampsia. Edited by: Ksenija Bogoeva-Kostovska Citation: Omertayeva D, Muravlyova L, Ponomaryova O, Molotov-Luchanskyi V, Bakirova R, Klyuev D, Mugazov M. The Level Assessment of Extracellular Nucleic Acids in the Blood of Pregnant Women with Chronic Hypertension with Superimposed Preeclampsia. Open Access Maced J Med Sci. 2020 Apr 20; 8(B):514-518. https://doi.org/10.3889/oamjms.2020.4180 Keywords: Extracellular nucleic acids; preeclampsia; arterial hypertension *Correspondence: Dinara Omertayeva, Department of Fundamental Medicine, National join-stock company Karaganda Medical University, Kazakhstan, 40 Gogolya Street, Karaganda 100008, Kazakhstan. E-mail: omertaevadinara@list.ru Received: 11-Dec-2019 Revised: 07-Feb-2020 Accepted: 07-Mar-2020 Copyright: © 2020 Dinara Omertayeva, Larissa Muravlyova, Olga Ponomaryova, Vilen Molotov-Luchanskyi, Ryszhan Bakirova, Dmitryi Klyuev, Miras Mugazov Funding: This research did not receive any fnancial support Competing Interests: The authors have declared that no competing interest exist Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution- NonCommercial 4.0 International License (CC BY-NC 4.0) Introduction Various types of hypertension are occurred during pregnancy: Preeclampsia, chronic arterial hypertension, chronic hypertension with superimposed preeclampsia, and gestational hypertension [1], [2], [3]. Preeclampsia and chronic arterial hypertension are one of the reasons of maternal and perinatal morbidity and mortality [2], [4], [5], [6]. The prevalence rate of preeclampsia in the world is 2.7–8.2% [3], [4], [5], [6]. Percentage of maternal mortality, caused by diseases of circulatory system, is 29.8 [7]. Preventive activities have low effciency, and screening tools are not always specifc, treatment is aimed at eliminating obvious clinical manifestations. The only effective treatment of these conditions is delivery [8], [9]. Thus, this complication of pregnancy remains a serious problem for doctors. Preeclampsia is a heterogeneous disorder, triggered by several different underlying mechanisms. There are more than 30 theories of the development of preeclampsia [8], [9], [10], [11]. One of the leading is the theory of endothelial dysfunction [12] and placental theory [9]. Violation of trophoblast invasion leads to decrease in oxygenation and, accordingly, to the formation of oxidative stress. Microvasculature’s damage in organs and tissues leads to oxidative stress and accumulation of reactive oxygen species (ROS). The result of lipid and protein peroxidation, activation of membrane phospholipases, mechanical (osmotic) stretching, and adsorption of foreign proteins are destruction of cells’ structural and functional properties [8], [9], [11]. At present, extracellular nucleic acids (ecNA) have a great interest [13], [14], [15], [16]. Extracellular nucleic acids can exist in free form or can be absorbed on blood cells or as a part of vesicles [15], [16], [17], [18]. Usually, the amount of circulating DNA is very low in healthy people. The growth of circulating DNA is observed in patients with