Research Article The Effectiveness of Mini Primer STR CODIS in DNA Degradation as the Effect of High-Temperature Exposure Ahmad Yudianto 1,2,3 and Fery Setiawan 2 1 Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Airlangga, Indonesia 2 Forensics Study Program, School of Postgraduate, Universitas Airlangga, Indonesia 3 Human Genetics Study Group-Institute of Tropical Disease, Universitas Airlangga, Indonesia Correspondence should be addressed to Ahmad Yudianto; yudi4n6sby@yahoo.co.id Received 22 July 2020; Accepted 10 December 2020; Published 24 December 2020 Academic Editor: Corina Danciu Copyright © 2020 Ahmad Yudianto and Fery Setiawan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. More and more today, forensic identification through deoxyribonucleic acid (DNA) examination has achieved greater recognition in supporting Indonesia’s law enforcement. Such examination is to determine the origin of a child, paternity cases, genealogical relation, or identifying unknown crime victims. However, along with the development of this DNA material examination, problems arise. DNA undergoes a degradation, commonly known as degraded DNA, which is one of the serious issues frequently encountered by forensic and DNA experts. Some forensic DNA experts take one of the alternatives to overcome this issue by implementing a mini primer set that is through a method to reduce the size of STR assays on DNA core locus examination. Methods. In this study, the writers conduct research using the mini primers of CSF1PO, FGA, and D21S11 of the molar teeth exposed to 500 ° C temperature for 20 and 30 minutes and 750 ° C for the same amount of time.Result. The findings show the DNA contents of molar teeth significantly (p <0:05) decreased as the effect of high-temperature exposure. PCR result visualization shows CSF1PO is the only locus detected with mini primer exposed to 750 ° C temperature for 30 minutes (the highest exposure during this research). Conclusions. This finding suggests that this locus is potential in examining identification through DNA analysis, especially on a degraded condition as the effect of high-temperature exposure. Besides, this could accelerate the identification process especially on mass disaster events or criminal cases. 1. Introduction More and more today, forensic identification through deoxy- ribonucleic acid (DNA) examination has achieved greater recognition in supporting Indonesia’s law enforcement. Such examination is to determine the origin of a child, paternity cases, genealogical relation, or identifying unknown crime victims. This has been proven by the recognition of the exam- ination invented by Sir Alec Jeffrey as one of the shreds of evidence in both judicial court and religion court since 1997. DNA identification played a significant role in identify- ing the victims of the Bali bombing back in 2002 [1, 2]. However, along with the development of DNA material examination, problems arise. One of the serious problems encountered by forensic DNA and other experts in this field is a DNA in degraded condition or commonly known as a degraded DNA [3, 4]. Some forensic DNA experts take one of the alternatives to overcome this issue by implementing a mini primer set that is through a method to reduce the size of Short Tandem Repeat (STR) assays on DNA core locus examination [5]. This research applied the average temperature of 500 ° C as treatment, the same as that in the previous research con- ducted by Thanakum (1999). Besides, the 750 ° C was applied, the same temperature as the research conducted by Sosiawan (2007) with 20 minutes duration, the longest duration of Thanakum’s research, and 30 minutes duration, the longest duration of Sosiawan’s [6, 7]. Nevertheless, until recently, there is no specific research on the effectiveness of the mini primer set to use as an alter- native in forensic DNA identification using degraded DNA, especially on the DNA core. It is important to determine Hindawi Analytical Cellular Pathology Volume 2020, Article ID 2417693, 6 pages https://doi.org/10.1155/2020/2417693