Plant Archives Volume 20 No. 2, 2020 pp. 7854-7856 e-ISSN:2581-6063 (online), ISSN:0972-5210 BIOSYNTHESIS OF ZNO NANOPARTICLE IN PRESENCE OF RED ONION EXTRACT Neeran Obied Jasim 1 * and Nabel Kadum Abd-Ali 2 1* College of science, University of AL-Qadisiyah, Iraq. 2 College of engineering, University of AL-Qadisiyah, Iraq. Abstract Nanoparticles of ZnO were synthesized by eco-friendly method by using red onion extract which using as a reducing agent. Characterized of these particles were investigated by UV-Vis spectroscopy, scanning electron microscope (SEM) and FTIR analyze. Also activity of ZnO nano-sized studied by tested against of yeast ( Cryptococcus neoformans) and bacteria (Bacillus subtillis). Results of Uv-Vis spectrum show, ZnO nanoparticles have absorption band at 370nm and SEM images were shows spherical like structure. Antimicrobial activity tests appear nano ZnO have effects on growth of microorganisms were tested. Key words : ZnO, Biosynthesis, C. neoformans, B. subtilis, nanoparticles. Introduction In recent year the development of nanotechnology has led to the growing of our ability to deal with engineering material and nano scale material and manufactured it (Vennila & Jesurani, 2017) Nanoparticles are characterized by different qualities of molecules when they are large size ,where possessing magnetic ,electronic and optical properties make them behave differently . Among these metal nanoparticles are promising because they have remarkable antimicrobial properties ,these due to their large surface ratio to their size (Saputra & Yulizar, 2017). From nanoparticles that have become famous among researchers, ZnO nanoparticles due to its various applications (Swati, et al., 2015) Zinc oxide is an interesting metal oxide because it can exist In different forms,it can be synthesis by different methods .These methods may be chemical such as sol-gel technique and other ways ,biological methods which using biological agents such as plant extracts are a promising alternative to conventional method of chemical synthesis (Swati, et al., 2015; Phindile, 2012) Many research has shown that, plant extracts, can produce nanoparticles through biological pathways (Lizabeth & Marym, 2015; Awwad, et al., 2013). Thus the current work shows that red onion extract on the synthesis of zinc oxide nanoparticles. Only some reports green synthesis of nanoparticles zinc oxide using extracts but this is the first time to report the detection of red onion extract and characterizations techniques. Materials and Methods Prepare extract: 50 grams of red onion fruits were washed twice or more well with distilled water to remove the dust particles then Finely chopped and crushed using a mortar and pestle then 100ml of double distilled water. The yellow extract was filtered through the filter paper Maman No. 1. Fungal Strain: Cryptococcus neoformans and bacillus subtilis which obtain from laboratory of microbiology of Science College /university of AL- Qadisiyah. Biosynthesis of Zinc Oxide nanoparticles: 1Mm of Zinc Acetate Zn (O‚ CCHƒ )‚ was dissolved in 100 ml de-ionized water under magnetic stirring at room temperature (1200 rpm). After obtaining a solution, 1-20 ml of an aqueous solution of red onion extracts was added drop by drop in zinc acetate solution Change in white to brown. Leave the mixture under stirring for 2 minutes stirring vigorously. The suspended particles were purified by dispersing insterilized distilled water and centrifuged 4 times. Afterwards, the nanoparticles were washed with (DDW) and dried at 100°C. *Author for correspondence : E-mail : neran.jasim@qu.edu.iq