Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Tue, 01 Jan 2019 23:08:08 Nocardiopsis oceani sp. nov. and Nocardiopsis nanhaiensis sp. nov., actinomycetes isolated from marine sediment of the South China Sea Hua-Qi Pan, 1 3 Dao-Feng Zhang, 2,3 3 Li Li, 4 Zhao Jiang, 3 Juan Cheng, 3 Yong-Guang Zhang, 4 Hong-Fei Wang, 4 Jiang-Chun Hu 1 and Wen-Jun Li 2,3,4 Correspondence Wen-Jun Li liwenjun3@mail.sysu.edu.cn Jiang-Chun Hu hujc@iae.ac.cn 1 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, PR China 2 State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PR China 3 Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China 4 Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, U ¨ rumqi 830011, PR China Two actinomycete strains, designated 10A08A T and 10A08B T , were isolated from marine sediment samples of the South China Sea and their taxonomic positions were determined by a polyphasic approach. The two Gram-stain-positive, aerobic strains produced branched substrate mycelium and aerial hyphae, and no diffusible pigment was produced in the media tested. At maturity, spore chains were formed on aerial hyphae and all mycelium fragmented with age. Whole-cell hydrolysates of both strains contained meso-diaminopimelic acid and no diagnostic sugars. Their predominant menaquinones (.10 %) were MK-9(H 4 ), MK-9(H 6 ) and MK-10(H 6 ) for strain 10A08A T and MK-9(H 4 ), MK-9(H 6 ), MK-10(H 4 ) and MK-10(H 6 ) for strain 10A08B T . The polar lipids detected from the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and unknown phosphoglycolipids and phospholipids. The major fatty acids (.10 %) of both strains were iso-C 16 : 0 and summed feature 4 (iso- C 17 : 1 I and/or anteiso-C 17 : 1 B). The genomic DNA G+C contents of strains 10A08A T and 10A08B T were 70.9 and 71.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarities, the two strains were shown to be most closely related to species of the genus Nocardiopsis. DNA–DNA hybridization relatedness values of ,70 % between these two isolates and their closest neighbour, Nocardiopsis terrae YIM 90022 T , and between the two strains supported the conclusion that they represent two novel species. Based on phylogenetic analysis and phenotypic and genotypic data, it is concluded that the two isolates belong to the genus Nocardiopsis, and the names Nocardiopsis oceani sp. nov. (type strain 10A08A T 5DSM 45931 T 5BCRC 16951 T ) and Nocardiopsis nanhaiensis sp. nov. (type strain 10A08B T 5 CGMCC 47227 T 5BCRC 16952 T ) are proposed. The genus Nocardiopsis was proposed by Meyer (1976) on the basis of chemotaxonomic and morphological characteristics, and is accommodated in the family Nocar- diopsaceae of the suborder Streptosporangineae. Members of the genus Nocardiopsis are distributed ubiquitously in natural environments (Kroppenstedt & Evtushenko, 2002). The natural habitat of most described strains is soil (Kroppenstedt & Evtushenko, 2002), but strains have also been isolated from marine environments (Sabry et al., 2004; Chen et al., 2009; Fang et al., 2011; Li et al., 2012), plant rhizosphere soil (Hamedi et al., 2010), plant tissue (Qin et al., 2009), animal guts (Vasanthi & Hoti, 1992), indoor environments (Peltola et al., 2001), the atmosphere of a composting facility (Ka ¨mpfer et al., 2002), clinical 3These authors contributed equally to this work. Abbreviation: DAP, diaminopimelic acid. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 10A08A T and 10A08B T are KF270094 and KF270095. Three supplementary figures and a supplementary table are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2015), 65, 3384–3391 DOI 10.1099/ijsem.0.000425 000425 G 2015 IUMS Printed in Great Britain 3384