Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Sat, 03 Nov 2018 01:11:25 Saccharomonospora paurometabolica sp. nov., a moderately halophilic actinomycete isolated from soil in China Wen-Jun Li, 1 Shu-Kun Tang, 1 Erko Stackebrandt, 2 Reiner M. Kroppenstedt, 2 Peter Schumann, 2 Li-Hua Xu 1 and Cheng-Lin Jiang 1 Correspondence Cheng-Lin Jiang lihxu@ynu.edu.cn or liact@yahoo.com 1 The Key Laboratory for Microbial Resources of the Ministry of Education, P. R. China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, China, 650091 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, 38124 Braunschweig, Germany A novel, moderately halophilic actinomycete, strain YIM 90007 T , was isolated from a soil sample collected from the Xinjiang Province, China, and characterized. The optimum growth temperature of the strain was between 35 and 37 6C and growth occurred optimally in 10 % (w/v) NaCl. The cell wall of strain YIM 90007 T contained meso-diaminopimelic acid. Whole-cell sugars were galactose, arabinose and ribose. The principal menaquinone was MK-9(H 4 ), while MK-9(H 2 ) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphospha- tidylethanolamine. The predominant cellular fatty acids were of the iso- and anteiso-branched and unbranched types; significant amounts of 2-hydroxy fatty acids were also found but 10-methyl-branched fatty acids were missing. The DNA G+C content of strain YIM 900007 T was 71 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed strain YIM 90007 T to be closely related to Saccharomonospora halophila (98?7 % similarity). DNA–DNA hybridization revealed a relatedness of 53?8 % between strain YIM 90007 T and S. halophila DSM 44411 T . Based on physiological and biochemical characteristics, phylogenetic analysis (based on 16S rRNA gene sequences) and DNA–DNA relatedness, it is concluded that strain YIM 90007 T represents a novel species of the genus Saccharomonospora, for which the name Saccharomonospora paurometabolica (type strain YIM 90007 T =CCTCC AA001018 T =CCRC 16315 T =DSM 44619 T ) is proposed. The genus Saccharomonospora (Nonomura & Ohara, 1971) was created for actinomycetes producing predominantly single spores on aerial hyphae. The cell wall contains meso- diaminopimelic acid together with the sugars arabinose and galactose. At the time of writing, the genus embraces six species, namely, Saccharomonospora viridis (Nonomura & Ohara, 1971), Saccharomonospora azurea (Hu, 1987), Saccharomonospora glauca (Greiner-Mai et al., 1988), Saccha- romonospora cyanea (Hu et al., 1988), Saccharomonospora xinjiangensis (Jin et al., 1998) and Saccharomonospora halophila (Al-Zarban et al., 2002); only the latter species is halophilic. During a study on halophilic actinomycetes, a halophilic strain, YIM 90007 T , was isolated from one soil sample from the Xinjiang Province, in the west of China. Strain YIM 90007 T was isolated on modified glycerol/ asparagine agar (Shirling & Gottlieb, 1966) [ISP5 medium containing 20 % (w/v) NaCl] and incubated at 28 u C for about 4 weeks. The strain was maintained on ISP2 and ISP5 agar slants containing 10 % NaCl at 4 u C and as glycerol suspensions (20 %, v/v) at 220 uC. Biomass for chemical and molecular systematic studies was obtained by growing in shake flasks (about 150 r.p.m.) of ISP2 medium broth containing 10 % NaCl at 28 uC for 1 week. Cultural charac- teristics were determined after 4 weeks at 28 u C by methods used in the International Streptomyces Project (ISP) (Shirling & Gottlieb, 1966). Morphological properties, spores and mycelia were examined by light microscopy (Olympus microscope BH-2) and scanning electron microscopy with a JEOL, model JSM5600LV. Media and procedures used for Published online ahead of print on 21 March 2003 as DOI 10.1099/ ijs.0.02633-0. The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of Saccharomonospora paurometabolica YIM 90007 T is AF540959. 02633 G 2003 IUMS Printed in Great Britain 1591 International Journal of Systematic and Evolutionary Microbiology (2003), 53, 1591–1594 DOI 10.1099/ijs.0.02633-0