Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Fri, 07 Dec 2018 16:36:33 Yimella lutea gen. nov., sp. nov., a novel actinobacterium of the family Dermacoccaceae Shu-Kun Tang, 1 3 Jin-Yuan Wu, 1 3 Yun Wang, 2 Peter Schumann 3 and Wen-Jun Li 1,4 Correspondence Wen-Jun Li wjli@ynu.edu.cn or liact@hotmail.com 1 The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China 2 Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang 830091, PR China 3 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany 4 Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China A Gram-stain-positive, coccoid, non-motile, halotolerant actinobacterium, designated YIM 45900 T , was found as a contaminant on an agar plate in the laboratory of Yunnan Institute of Microbiology, China. The peptidoglycan type was A4a with an L-Lys–L-Ser–D-Asp interpeptide bridge. The cell-wall sugars contained galactose and fucose. The predominant menaquinone was MK-8(H 4 ). The major fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 and anteiso-C 17 : 0 . The polar lipids contained diphosphatidylglycerol, phosphatidylinositol, a glucosamine-containing phospholipid and an unknown phospholipid. The DNA G+C content was 65.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism falls within the radius of the suborder Micrococcineae and its closest phylogenetic neighbours are the genera of the family Dermacoccaceae. Strain YIM 45900 T showed 16S rRNA gene sequences similarity values of 93.1–95.9 % with members of the genera Dermacoccus, Demetria and Kytococcus. On the basis of the phylogenetic and phenotypic characteristics of the actinobacterium, a novel genus and species, Yimella lutea gen. nov., sp. nov., are proposed. The type strain of Yimella lutea is YIM 45900 T (5DSM 19828 T 5KCTC 19231 T 5CCTCC AB 207007 T ). The family Dermacoccaceae was first proposed by Stackebrandt & Schumann (2000) and its pattern of 16S rRNA signature nucleotides was emended by Zhi et al. (2009). At the time of writing, the family Dermacoccaceae comprises the genera Dermacoccus (Stackebrandt et al., 1995), Demetria (Groth et al., 1997) and Kytococcus (Stackebrandt et al., 1995). Most strains have been isolated from human skin. They are Gram-positive, aerobic, non- encapsulated, non-endospore-forming, catalase-positive, non-halophilic, coccoid actinobacteria and the peptidogly- can type is variation A4a. Strain YIM 45900 T , a Gram-stain-positive, coccoid, non- motile, halotolerant actinobacterium, was found in the laboratory of Yunnan Institute of Microbiology, China, as a contaminant on a modified ISP medium 5 (Shirling & Gottlieb, 1966) agar plate (containing l 21 distilled water: 1g L-asparagine, 10 g glycerol, 5g yeast extract, 1g K 2 HPO 4 , 5 g KNO 3 , 50 g NaCl, 15 g agar; final pH 7.5). NaCl was sterilized separately before being added to the medium. Strain YIM 45900 T was maintained on ISP 2 (Shirling & Gottlieb, 1966) agar slants at 4 u C and in 20 % (w/v) glycerol suspensions at 220 u C. Biomass for chemical and molecular studies was obtained by cultivation in shaken flasks (about 150 r.p.m.) using ISP 2 broth (pH 7.5) at 28 u C for about 1 week. Cell morphology was determined on cultures grown for 6, 12, 24, 48 and 72 h on ISP 2 agar medium at 28 u C. Gram staining was carried out by the standard Gram reaction and was confirmed by using KOH lysis (Cerny, 1978). Spore formation was determined by staining with malachite green. Cell motility was confirmed by the development of turbidity throughout a tube containing semisolid medium (Leifson, 1960). Morphological characteristics of the strain were observed by light microscopy (model BH 2; Olympus) 3These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 45900 T is FJ528304. International Journal of Systematic and Evolutionary Microbiology (2010), 60, 659–663 DOI 10.1099/ijs.0.013920-0 013920 G 2010 IUMS Printed in Great Britain 659