~7~ International Journal of Fisheries and Aquatic Studies 2016; 4(3): 07-11 ISSN: 2347-5129 (ICV-Poland) Impact Value: 5.62 (GIF) Impact Factor: 0.352 IJFAS 2016; 4(3): 07-11 © 2016 IJFAS www.fisheriesjournal.com Received: 03-03-2016 Accepted: 05-04-2016 Md Rezwanul Hasan Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore- 7408, Bangladesh. Md Sherazul Islam Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore- 7408, Bangladesh. Aeyrin Afroz Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore- 7408, Bangladesh. Ponkoj Bahadur Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore- 7408, Bangladesh. Sharmin Akter Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore- 7408, Bangladesh. Correspondence Md Sherazul Islam Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore- 7408, Bangladesh. Captive breeding of Striped Spiny Eel, Mastacembelus pancalus (Hamilton, 1822) considering the various hormonal responses Md Rezwanul Hasan, Md Sherazul Islam, Aeyrin Afroz, Ponkoj Bahadur, Sharmin Akter Abstract The spawning performance of spiny eel, M. pancalus was investigated by using different hormones. The water quality parameters were within optimum ranges such as average temperature, DO and pH were 29.13 ºC, 4.19 mg/L and 8.32 respectively. Hormones were administered into females in two doses; a lower priming dose followed a 6 h interval by a higher resolving dose. In first dose, the female were injected ovaprim, HCG and PG at 0.25 ml/kg, 200 IU/kg and 30 mg/kg of body weight respectively. The second dose was applied to both male and female and female received double amount of hormone of the first dose. The male were administered a single dose at 0.5 ml/kg, 400 IU/kg and 60 mg/kg of ovaprim, HCG and PG respectively. It is indicated that the use of ovaprim was more effective in spawning (100%), fertilization (75%) and hatching (55%) of M. pancalus compared to the other stimulators. Keywords: Mastacembelus pancalus, induced breeding, hormones, spawning 1. Introduction The Striped spiny eel (Mastacembelus pancalus) is one of the common species of fishes of the family Mastacembelidae and important eel fish in Bangladesh with great demand as a good traditional table fish [1] . Locally the fish is called guchi, baim, turi or chirka. However, very commonly known as guchi baim. The species is distributed in India, Pakistan, Bangladesh [22] and Nepal [8] . Guchi baim was available in rivers, canals, beels and inundated fields in the past throughout Bangladesh but presently it is under threat due to tremendous loss of natural habitat [14] . According to IUCN [10] the fish is considered to be a critically endangered in the country. The fish is not yet incorporated in inland culture system. The main reasons behind this are insufficient information on the captive induced breeding, inadequateness of natural fry and absence of commercial hatchery production. Considering the importance of the species, adequate research in this field is required. Notable works have been done on striped spiny eel such as Talwar and Jhingran [22] have studied on the age and growth; Karim and Hossain [11] , Vettath et al. [23] , Saha et al. [17] and Pathak et al. [12] have studied on the reproductive biology; Alam et al. [3] and Rahman et al. [14] have studied on the induced breeding of M. pancalus with different doses of PG; Das and Kalita [6] have studied on the induced captive breeding of Macrognathus aculeatus with different doses of ovaprim. But so far no works have been done on the induced breeding of M. pancalus with different hormones. It is necessary to undertake proper study to identify and characterized the breeding protocol of the fish. The research aim was to establish an induced breeding protocol considering the various hormonal responses of M. pancalus. 2. Materials and Methods The major part of the experiment was conducted at the Laboratory, Department of Fisheries and Marine Bioscience, Jessore University of Science and Technology (JUST), Bangladesh. The experiment was done from 10 March to 30 June 2014. 2.1 Brood fish collection and rearing Samples of M. pancalus for induced breeding were collected from Jhapa boar, Jessore and were kept in the previously prepared experimental pond of the university. For successful