The genus Bothrops Wagler, 1824, comprises 45 species, nine of which (B. asper, B. atrox, B. ayerbei, B. bilineatus, B. brazili, B. pulchra, B. punctatus, B. taeniatus, B. venezuelensis) occur in Colombia (Uetz et al., 2020). Bothrops punctatus (García, 1896) is easily distinguishable from its congeners in Colombia by presenting a pale-coloured, distally prehensile tail in adults (García, 1896; Campbell and Lamar, 2004; Fenwick et al., 2009), which seems to be related to its semi-arboreal existence (Campbell and Lamar, 2004). In addition, B. punctatus can be distinguished from B. asper, which is found in sympatry in some regions (e.g., Vargas-Salinas and Bolaños-Lizalda, 1999), by having rounded dorsal spots and an ornate pattern on the top of the head. Bothrops punctatus is distributed in the biogeographic provinces of Chocó-Darién, Magdalena, and Western Ecuador (sensu Morrone, 2014), from southeastern Panama (Darién Gap) through Colombia to northwestern Ecuador (Pérez-Santos and Moreno, 1988; Campbell and Lamar, 2004; Uetz et al., 2020). In Colombia, it is distributed along the Pacifc Coast and in both the Cordillera Occidental and the Cordillera Central, in the departments of Antioquia, Cauca, Chocó, and Valle del Cauca, occurring in humid, very humid, and rainforests of tropical, pre-montane, and montane regions, and in pasturelands from elevations of 9–2300 m (Pérez-Santos and Moreno, 1988; Vargas-Salinas and Bolaños-Lizalda, 1999; Campbell and Lamar, 2004; Daza-Rojas et al., 2005; Arteaga et al., 2013; Ospina- Larrea, 2017; Fig. 1, Appendix 1). During a herpetological survey in the Parque Nacional Natural Selva de Florencia (hereafter PNNSF), Samaná Municipality, Caldas Department, Colombia (Fig. 1), we recorded four juvenile B. punctatus on the bank of San Antonio Stream in Vereda San Lucas (5.4981°N, 75.0522°W, elevation 1216 m; datum WGS84). One individual (snout–vent length 299 mm; tail length 59 mm; Fig. 2A) was collected on the ground near the stream on 22 April 2018 at 15:56 h. The specimen was deposited in the reptile collection of the Museo de Historia Natural Universidad de Caldas (MHN- UCa) under accession number MHN-UCa 0327. The other three individuals were recorded on 23 April 2018 between 19:00 and 19:40 h along the same stream where the individual collected the previous night was observed. These individuals were observed on rocks (Fig. 2B) or fallen tree branches. The observations in the PNNSF constitute the frst record of this species from Caldas Department, extending the species’ geographic range in Colombia southwards along the eastern versant of the Cordillera Central in the Magdalena River Valley, specifcally by ca. 60 km to the southwest of the nearest previous record in San Luis Municipality, Antioquia Department (6.0333°N, 74.9833°W; Daza-Rojas et al., 2005). In the PNNSF, B. punctatus is sympatric with B. asper and Bothriechis schlegelii. Bothrops punctatus is mainly distributed in the Chocó- Darién biogeographic province (Campbell and Lamar, 2004; Daza-Rojas et al., 2005; Ospina-Larrea, 2017), and distributions spanning Chocó-Darién and Magdalena biogeographic province have also been observed in other reptile species, including Diploglossus monotropis, Imantodes inornatus, Loxopholis southi, and Micrurus dumerilii (Gutiérrez-Cárdenas and Arredondo-Salgar, 2007; Díaz-Ayala et al., 2015, 2018; Rojas-Morales et al., 2016). The geographic distribution pattern of B. punctatus, as well as that of the other species, shows a faunal affnity between these two regions, as discussed by Hernández-Camacho et al. (1992). Our record for B. punctatus extends further south than previously known in the mid-Magdalena River basin (Daza-Rojas et al., Herpetology Notes, volume 14: 1127-1131 (2021) (published online on 20 August 2021) First record of the Spotted Lancehead, Bothrops punctatus (García, 1896), from the Department of Caldas, Colombia Jhoana A. Santana-Londoño 1,* , Juan P. Moncada-Vélez 1 , Juan C. Gallego-Ramírez 1 , Viviana A. Ramírez-Castaño 2 , and Paul D.A. Gutiérrez-Cárdenas 1 1 Grupo de Ecología y Diversidad de Anfbios y Reptiles, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 #26-10, A.A. 275, Manizales, Colombia. 2 Grupo de Investigación en Biodiversidad y Recursos Naturales, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 #26-10, A.A. 275, Manizales, Colombia. * Corresponding author. E-mail: jsantanalondono@gmail.com © 2021 by Herpetology Notes. Open Access by CC BY-NC-ND 4.0.