Choroby rozrostowe krwi – analiza transkryptomu z zastosowaniem mikromacierzy DNA Luiza Handschuh 1,2 , Krzysztof Lewandowski 1 , Maciej KaŸmierczak 1 , Mieczys³aw Komarnicki 1 , Marek Figlerowicz 2 1 Katedra i Klinika Hematologii i Chorób Rozrostowych Uk³adu Krwiotwórczego, Uniwersytet Medyczny im. K. Marcinkowskiego, Poznañ 2 Instytut Chemii Bioorganicznej, Polska Akademia Nauk, Poznañ Blood proliferative diseases – application of the DNA microarrays for transcriptome analysis Summary Hematopoiesis is a complex process precisely regulated by a wide spectrum of cooperating factors. Dysfunction of hematopoietic cell proliferation, differ- entiation or maturation usually leads to the malignant transformation. The DNA microarray-based transcriptome analysis helped to revise the traditional classifi- cation of hematological disorders, predict their outcome, test potential thera- peutic agents and better understand basic mechanisms underlying cancer origin and development. Here, the results of gene expression profiling in myelo- and lymphoproliferative diseases such as leukemia, lymphoma and myelodysplastic syndromes, are presented. Two microarray technologies were applied in this area of research: Affymetrix gene chips and cDNA microarrays. Among them, Lymphochip is a prominent example of a specialized cDNA microarray tool de- signed to investigate gene expression in the immunological system and hemato- logical diseases. It seems that typical problems connected with microarray re- sults analysis – small number of patients, loss of reproducibility can be over- come by increasing the number of samples and application of identical proto- cols, equipment and reagents in different laboratories. Key words: proliferative diseases, leukemia, lymphoma, mielodysplastic syndromes, transcriptome, gene expression profiling, microarrays. PRACE PRZEGL¥DOWE Adres do korespondencji Luiza Handschuh, Instytut Chemii Bioorganicznej, Polska Akademia Nauk, Centrum Doskona³oœci CENAT, ul. Noskowskiego 12/14, 61-704 Poznañ; e-mail: luizahan@ibch.poznan.pl 3 (86) 22–43 2009