Zeinat. W. Ribat, Int. J. Res. Pharm. Sci., 10(3), 1910-1913 ORIGINAL ARTICLE INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACEUTICAL SCIENCES Published by JK Welfare & Pharmascope Foundation Journal Home Page: www.pharmascope.org/ijrps Effect of vitex agnus castus fruits methanol extract against murine mammary adenocarcinoma cell line (amn3) and rat embryonic ৎibroblast normal cell line (ref) Zeinat. W. Ribat *1 , Hayder B Sahib 2 , Amjed Haseeb Khamees 3 , Ahmed M. Al-Shammari 4 1 Department of Pharmacology and Therapeutics, College of Medicine, AL Nahrain University, Iraq 2 College of Pharmacy, Al-Nahrain University, Iraq 3 Department of Pharmacognosy, College of Pharmacy, University of Baghdad, Iraq 4 Iraqi Centre for Cancer and Medical Genetic Researches, Al-Mustansiriyah University, Iraq Article History: Received on: 10.03.2019 Revised on: 15.06.2019 Accepted on: 20.06.2019 Keywords: Vitex agnus castus, mammary gland adenocarcinoma cell line, rat embryonic ϑibroblast cell line, antiproliferative activity ABSTRACT Vitex agnus castus showed antiproliferative activity in several previous stud- ies. Angiogenesis is one of the targets in the remediation of cancer. This study aimed to demonstrate the effect of methanol fruits extract of vitex agnus cas- tus on mouse mammary gland adenocarcinoma cell line and rat embryonic ϑibroblast cell line. The cell lines used in this study were obtained from tis- sue culture unit/ Iraqi Center for Cancer and Medical Genetic Researches, Al- Mustansiriyah University was maintained in RPMI- 1640 tissue media after preparing from 10% fetal calf serum, antibiotics solution and other materi- als to make complete growth medium. Serial solutions of vitex agnus castus, methanol crude extract have been tested on 10*4 of AMN3 and REF in each well of 96 well plates. The results of the current study showed that the con- centration that inhibits ϑifty percent of cell line after 72 hours of the experi- ment (IC50) was 129 ug/ml for AMN3 and 1324ug/ml for REF cell line. The antioxidant activity of Vitex agnus castus may indicate the proliferation inhibi- tion activity of Vitex agnus castus methanol extract. The study concluded that this extract might be of beneϑit if used in combination with other anti-cancer drugs as adjuvant therapy. * Corresponding Author Name: Zeinat. W. Ribat Phone: Email: zeinatwathiq@gmail.com ISSN: 0975-7538 DOI: https://doi.org/10.26452/ijrps.v10i3.1393 Production and Hosted by Pharmascope.org © 2019 | All rights reserved. INTRODUCTION Vitex agnus castus, also called Chaste Tree, Abra- hams balm, chaste berry, hemp tree, kaff Maryam, lilac chaste tree, monks pepper, shajerat Ebrahim, vitex, wild lavender (W.H.O., 1999) .Regional to the Mediterranean area, European and Asia; it is widely distributed in the Middle East, southern United State and Canada, it is plowed in warm temperate and sub-tropical regions of the world and gained mostly from Mediterranean countries especially Albania and Morocco, it prefers full sun to partial shade in a well-drained soil (Rani and Sharma, 2013). Histor- ically, VAC found in the ancient as an ofϑicial plant, and it is named in the works of Hippocrates, Diosc- crides, Theophrastus, and others. The ϑirst detailed medicinal hints came from Hippocrates, mentioning its use not only for injuries, inϑlammation and spleen enlargement, but also the leaves in wine used for the ϑlow of blood and for the expulsion after birth (Ger- hard and R.M., 1938). Several common uses include premenstrual syndrome (Cerqueira et al., 2017), ab- normal uterine bleeding disorders, and mastody- nia (Seidlova-Wuttke and Wuttke, 2017). Many sci- 1910 © International Journal of Research in Pharmaceutical Sciences