Research Article Development of Radiofrequency Ablation Generator and Balloon-Based Catheter for Microendoluminal Thin-Layer Ablation Therapy Using the Rat Duodenum as a Model of Low-Impedance Tissue Jana Nociarova , 1 Marek Novak , 2 Jan Polak , 1,3 Jan Hrudka , 4 Stefan Porubsky , 5 Michal Koc , 3 Jozef Rosina , 2 Aleksandr N. Grebenyuk , 6 Radek Sery , 7 Robert Gurlich , 8 and Jan Hajer 1 1 Department of Internal Medicine, ird Faculty of Medicine, Charles University, University Hospital Kralovske Vinohrady, Prague 100 34, Czech Republic 2 Department of Medical Biophysics and Medical Informatics, ird Faculty of Medicine, Charles University, Prague 100 00, Czech Republic 3 Department of Pathophysiology, ird Faculty of Medicine, Charles University, Prague 100 00, Czech Republic 4 Department of Pathology, ird Faculty of Medicine, Charles University, University Hospital Kralovske Vinohrady, Prague 100 34, Czech Republic 5 Institute of Pathology, University Medical Center of the Johannes Gutenberg University in Mainz, Mainz 55 131, Germany 6 Department of Health Protection and Disaster Medicine, Pavlov First State Medical University of St. Petersburg, St. Petersburg 197 022, Russia 7 Centre of Toxicology and Health Safety, National Institute of Public Health, Prague 100 00, Czech Republic 8 Department of General Surgery, ird Faculty of Medicine, Charles University, University Hospital Kralovske Vinohrady, Prague 100 34, Czech Republic Correspondence should be addressed to Jana Nociarova; janociarova@gmail.com Received 18 March 2021; Revised 7 September 2021; Accepted 14 September 2021; Published 24 September 2021 Academic Editor: Ayush Dogra Copyright©2021JanaNociarovaetal.isisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Radiofrequency ablation (RFA) is a routinely used, safe, and effective method for the tissue destruction. Often, in case of its application in malignant conditions, the extent of tissue destruction is insufficient due to the size of the target lesion, as well as due to the risk of heat-induced damage to the surrounding organs. Nevertheless, there are conditions requiring superficial precise- depth ablation with preservation of deeper layers. ese are represented, for example, by mucosal resurfacing in case of Barrett’s esophagus or treatment of recurrent mucosal bleeding in case of chronic radiation proctitis. Recently, new indications for intraluminal RFA use emerged, especially in the pancreatobiliary tract. In the case of intraductal use of RFA (e.g., biliary and pancreatic tract), there are currently available rigid and needle tip catheters. An expandable balloon-based RFA catheter suitable for use in such small-diameter tubular organs could be of benefit due to possible increase of contact between the probe and the target tissue; however, to prevent excessive tissue damage, a compatible generator suitable for low-impedance catheter/tissue is essential. is project aimed to develop a radiofrequency ablation generator and bipolar balloon-based catheter optimized for the application in the conditions of low-impedance tissue and (micro)endoluminal environment. Subsequent evaluation of biological effect in vivo was performed using duodenal mucosa in Wistar rat representing conditions of endoluminal radiofrequency ablation of low-impedance tissue. Experiments confirming the safety and feasibility of RFA with our prototype devices were conducted. Hindawi Journal of Healthcare Engineering Volume 2021, Article ID 9986874, 12 pages https://doi.org/10.1155/2021/9986874