Gwetu et al., Pediat Therapeut 2013, 4:1
DOI: 10.4172/2161-0665.1000187
Open Access Research Article
Volume 4 • Issue 1 • 1000187
Pediat Therapeut
ISSN: 2161-0665 Pediatrics, an open access journal
Hemocue Validation for the Diagnosis of Anaemia in Children: A Semi-
Systematic Review
Gwetu TP*, Chhagan MK, Craib M and Kauchali S
Department of Public health, University of KwaZulu-Natal, Durban, South Africa
Abstract
Background: Numerous methods are endorsed for Haemoglobin (Hb) estimation and anaemia assessment. The
Hemocue is being introduced for routine use in clinics and hospitals in many developing nations. There are a number of
illustrative cases that establish a clinical connection readily, but these have mainly been with adults. This study seeks
tore view literature on the diagnostic accuracy of the Hemocue among children.
Methodology: This is a semi-systematic review of studies analysing the Hemocue device’s diagnostic accuracy
determining haemoglobin levels among children aged zero to ffteen years.
Results: 18 studies were included. The main fnding of this investigation is that the Hemocue system is a good
screening test, being sensitive and reliably projecting necessity of a full blood count. It is not likely that diagnosis of
a clinically signifcant condition can be overlooked by this investigation. Thus it would seem to be a useful method
to use for Hb screening in appropriate situations. The studies reviewed generally reported a difference between the
means obtained by Hemocue Laboratory analyser, though these did not reach statistical signifcance. A trend for
underestimation of Hb values was reported with most studies. The Hemocue had a sensitivity range of 75-91%,
specifcity range of 88-100% and positive predictive values ranging from 75-80% for the detection of anaemia. The
mean difference in Hb from paired samples ranged from 0.2- 0.35 g/dl (0.7%).
Conclusion: The Hemocue is comparable to usual laboratory methods for determination of Hb level in children.
It is well appropriate for use in care of healthy paediatric patients and children with hematologic disorders. A full blood
count is recommended when anaemia is identifed or in suspected non-anaemic iron defciency.
*Corresponding author: T P Gwetu, Department of Public health, Maternal and
Child health unit, University of Kwazulu-Natal, P/Bag 7 Congella, Durban, 4013,
South Africa, Tel: +2733 387 9562; E-mail: tgwetu@gmail.com
Received June 17, 2013; Accepted December 02, 2013; Published December
04, 2013
Citation: Gwetu TP, Chhagan MK, Craib M, Kauchali S (2013) Hemocue Validation
for the Diagnosis of Anaemia in Children: A Semi-Systematic Review. Pediat
Therapeut 4: 187. doi:10.4172/2161-0665.1000187
Copyright: © 2013 Gwetu TP, et al. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited.
Keywords: Anaemia; Diagnosis; Screening; Hemoglobinometry;
Accuracy; Precision
Introduction
Haemoglobin (Hb) concentration is the most consistent anaemia
indicator [1]. Most reliable methodologies for testing Hb concentration
require some equipment that may not be available in primary care sites.
Te Hemocue measures Hb level in undiluted capillary or venous blood.
It is easily transportable, needs very little blood for analysis, does not
require blood sample storage or refrigeration, gives immediate digital
results and can be battery operated. Te device seems appropriate for
feld surveys, anaemia surveillance at sentry sites and to evaluate the
impact of nutritional intervention programs. For example, until three
years ago the South African Blood Transfusion Service used only the
CuSO4 method for Hb measurement in potential blood donors. Now,
all of their sites have changed to the Hemocue system. Te Hemocue
is also being rolled out to clinics and hospitals of South Africa where
it has found widespread acceptance and is being used for rapid Hb
assessment of both adults and children. Tis study reviewed the validity
of the Hemocue compared to laboratory reference methods in children.
Methods
Inclusion criteria for studies
Two reviewers identifed studies, extracted data, assessed
methodological quality and compared results of studies analysing the
diagnostic accuracy of the Hemocue with comparisons made against
a laboratory based Hb analyser. Samples were population or hospital
based. We also included studies that assessed Hemocue test reliability.
Tis study focuses on anaemia detection in children from neonates
up to age ffeen years. Electronic databases such as PubMed, Medline
and Science Direct were explored using keywords such as Hemocue,
Hb tests, anaemia screening, reliability and validity. Dates ranged from
the early seventies when the Hemocue was invented up to January
2013. A semi-systematic literature review was applied; using two
thematically separated data collection forms. Articles were reviewed in
stages and by categorization. Available abstracts in English were all read
before deciding which relevant full text to retrieve. We scored studies
according to pre-determined criteria such as the evidence for accuracy,
precision and potential clinical usefulness (unpublished observations).
Te decision on which studies to include in this research was made afer
further considering available data from each study using a specifcally
formatted inclusion form. Te pre-designed inclusion form contained
data on study quality, design and characteristics such as location,
laboratory reference method used to assess Hb concentration, health
care setting, age restrictions, sample size and outcome. A systematic
review or meta-analysis would subsequently examine in more detail
the limits to and determinants of efcacy in the identifed studies and
provide an exhaustive summary of literature relevant to the research
question
Results
Description of Studies
35 studies were found which analysed Hemocue Hb testing in
children. Only 18 studies met the research criteria and were included
in this study. Tese studies used dissimilar Hemocue devices, statistical
P
e
d
i
a
t
ri
c
s
&
T
h
e
r
a
p
e
u
t
i
c
s
ISSN: 2161-0665
Pediatrics & Therapeutics