ABSTRACT This study examined the anti-oxidative effects of Riboceine and vitamin C supplementation in the amelioration of tissue damage that results from oxidative stressors in diabetic rats. Twenty-fve (25) mature Wistar rats (150- 180kg) were used for the study. They were randomly assigned into fve (5) groups of fve rats each: Normal Control (NC), Diabetic Mellitus(DM) (induced by Alloxan at 120mg/kg body weight i.p), Diabetic rats treated with 30mg/kg body weight of RiboCeine (DM+RibCys), Diabetic rats treated with 125 mg/kg body weight of Vitamin C (DM+VitC) and Diabetic rats treated with 30 mg/kg body weight of RiboCeine + 125mg/kg body weight of Vitamin C (DM+RibCys +VitC). Following period of 20-day treatment, all the rats were sacrifced and samples of pancreas, kidney and liver tissues were collected and assayed for changes in oxidative stress biomarkers: Glutathione (GSH), Catalase (CAT), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Glutathione s-transferase (GST) and Malondialdehyde (MDA) levels. The tissues were also examined for Histo-architectural changes. Data were analysed using One Way Analysis of Variance (ANOVA) followed by Fisher’s Least Signifcant Difference (LSD). A p-value of p < 0.05 was considered statistically signifcant. RibCys and DM+RibCys+VitC treated rats, showed statistically signifcant decrease in hyperglycaemia. However, RibCys dosed rats, showed no statistically signifcant effect. The DM rats showed statistically signifcant increased MDA and decreased CAT, SOD, GPx, GST levels in the tissues. The RibCys, VitC and DM+VitC+RibCys treated rats, showed statistically signifcant amelioration in increased MDA and decreased CAT, SOD, GPx, GST levels. Present results concluded that RibCys has ameliorative effects on diabetes induced-oxidative stress. Also, RibCys, VitC and VitC+RibCys have equally antioxidative effects on tissues characterized by biomarkers of oxidative stress. KEY WORDS: RIBOCEINE, VITAMIN C, DIABETES MELLITUS, OXIDATIVE STRESS, ALLOXAN, ANTIOXIDANTS. Modulatory Role of Riboceine and Vitamin C Supplementation on Altered Oxidative Stress Status of Alloxan-induced Diabetic Wistar Rats Akingbade Bamidele Thomas 1 , Ojieh Anthony Emeka 2 , Nwogueze Bartholomew Chukwuebuka 3 *, Omeru Oghenerukevwe 4 and Ebuwa Emmanuel Ikemefune 5 1 Department of Physiotherapy, Central Hospital, Benin City, Edo State, Nigeria. 2,4,5 Department of Human Physiology, Delta State University, Abraka, Delta State, Nigeria. 3 Department of Human Physiology, Evangel University Akaeze, Ebonyi State, Nigeria. Biotechnological Communication 2130 Biosc.Biotech.Res.Comm. Vol 13 No (4) Oct-Nov-Dec 2020 Pp 2130-2138 ARTICLE INFORMATION *Corresponding Author: bukasono123@gmail.com Received 17th Oct 2020 Accepted after revision 12th Dec 2020 Print ISSN: 0974-6455 Online ISSN: 2321-4007 CODEN: BBRCBA Thomson Reuters ISI Web of Science Clarivate Analytics USA and Crossref Indexed Journal NAAS Journal Score 2020 (4.31) SJIF: 2020 (7.728) A Society of Science and Nature Publication, Bhopal India 2020. All rights reserved Online Contents Available at: http//www.bbrc.in/ DOI: http://dx.doi.org/10.21786/bbrc/13.4/75 INTRODUCTION Diabetes mellitus (DM) describes a metabolic disorder with complex pathophysiology and multiple aetiology characterised by chronic hyperglycaemia and disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin action, or both leading to lethal consequences in patients (Yu et al, 2020). Free radical-mediated destruction of pancreatic beta cells