1 Supporting Information Rec. Nat. Prod. 11:6 (2017) 552-557 Chemical and Biological Investigation of Ochrosia elliptica Labill. Cultivated in Egypt Riham A. El-shiekh 1 , Dalia A. Al-Mahdy 1 , Mohamed S. Hifnawy 1 , Tzvetomira Tzanova 2 , Emilie Evain-Bana 2 , Stéphanie Philippot 2 , Denyse Bagrel 2 , Essam A. Abdelsattar 1, * 1 Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr El Aini st., P.B. 11562, Egypt, 2 Molecular Engineering Laboratory and Pharmacological Biochemistry (LIMBP), Jean Barriol Institute, University of Lorraine, Metz, France Table of Contents Page 1 Experimental 3 1.1 Plant materials 1.2 General Experimental Procedures 1.3 Chemicals and Reagents 3 3 3 1.4 Extraction and Isolation 3 1.5 Biological Evaluation 3 1.5.1 Cell lines and culture conditions 3 1.5.2 Cell viability determination (MTT assay) 3 1.5.3 Evaluation of CDC25s inhibitory potential 3 1.5.4 In-vitro antioxidant activity 4 1.5.5 Determination of the median lethal dose (LD50) 4 1.5.6 In-vivo anti-inflammatory activity 4 1.5.7 Statistical analysis 5 2 Spectroscopic data and characterization 5 S1: 1 H-NMR (400 MHz, CDCl 3 ) spectrum of Lupeol acetate (1) 6 S2: 13 C-NMR (100 MHz, CDCl 3 ) spectrum of Lupeol acetate (1) 7 S3: ESI-MS spectrum of Lupeol (2) 8 S4: 1 H-NMR (400 MHz, CDCl 3 ) spectrum of Lupeol (2) 9