Research Article
Modeling and Simulation of VEGF Receptors Recruitment
in Angiogenesis
A. Salvadori ,
1,2
V. Damioli,
1
C. Ravelli,
2,3
and S. Mitola
2,3
1
DIMI, Universit` a degli Studi di Brescia, Brescia 25123, Italy
2
Laboratory for Preventive and Personalized Medicine (MPP Lab), Universit` a degli Studi di Brescia, Brescia 25123, Italy
3
DMMT, Universit` a degli Studi di Brescia, Brescia 25123, Italy
Correspondence should be addressed to A. Salvadori; alberto.salvadori@unibs.it
Received 21 December 2017; Revised 3 May 2018; Accepted 6 May 2018; Published 26 August 2018
Academic Editor: Anna Vila
Copyright © 2018 A. Salvadori et al. Tis is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Angiogenesis, the process of new blood vessel formation from preexisting ones, plays a pivotal role in tumor growth. Vascular
endothelial growth factor receptor-2 (VEGFR2) is the main proangiogenic tyrosine kinase receptor expressed by endothelial
cells (ECs). VEGFR2 binds diferent ligands triggering vascular permeability and growth. VEGFR2-ligands accumulate in the
extracellular matrix (ECM) and induce the polarization of ECs as well as the relocation of VEGFR2 in the basal cell membrane
in contact with ECM. We propose here a multiphysical model to describe the dynamic of VEGFR2 on the plasma membrane. Te
governing equations for the relocation of VEGFR2 on the membrane stem from a rigorous thermodynamic setting, whereby strong
simplifying assumptions are here taken and discussed. Te multiphysics model is validated against experimental investigations.
1. Introduction
Vascular Endothelial Growth Factor Receptor-2 (VEGFR2)
is a proangiogenic receptor expressed on endothelial cells
(ECs) and is the main mediator of the angiogenic response.
Te interaction between VEGFR2 and extracellular ligands,
produced by tumor cells, is essential to cancer growth. Specif-
ically, ligand stimulation causes the relocation of VEGFR2
in the basal aspect in cells plated on ligand-enriched extra-
cellular matrix both in vitro and in vivo, and ultimately
receptors-ligands interaction activates the ECs division and
proliferation towards tumor cells. Upon release, growth
factors associate with the extracellular matrix and act as ECs
guidance during neo-vessel formation.
Receptor-ligand interactions have been extensively stud-
ied and mathematical models have been proposed. Some
concerned the estimation of the reaction rates for membrane-
bound reactants [1–3]; a few models with diferent level of
complexity account for adhesion receptor-ligand (as inte-
grins and fbronectin) kinetics, receptor-ligand densities, cell
rheology, and cytoskeletal force generation [4]. Only a few
investigations concerned specifcally VEGFR2 [5, 6].
Codesigned experiments and simulations for VEGFR2
have been recently developed, with biological fndings and
the predictive ability of the model extensively discussed in
[7]. Here, we profoundly describe the modeling of VEGFR2
recruitment in angiogenesis, detailing the thermodynamic
description, the weak formulation, and the algorithms for the
numerical solution.
Te mathematical model here proposed accounts for
difusion of VEGFR2 along the cellular membrane and for
ligands-receptors chemical reactions. It is framed in the
mechanics and thermodynamics of continua, following a
general description proposed in [8], and takes advantage
of successful descriptions of physically similar systems [9,
10]. Te efect of the cell deformation on the difusion-
reaction process on the membrane is here strongly simplifed,
surrogating the efects of the change in geometry on the
chemodifusive equations with a fctitious source term of
ligands, detailed in Section 2.2.
Te model stems from continuity equations (for mass,
energy, and entropy; see Section 2.1), standard chemical
kinetics, summarized in Section 2.6, thermodynamic restric-
tions, and constitutive specifcations, detailed in Section 2.4.
Hindawi
Mathematical Problems in Engineering
Volume 2018, Article ID 4705472, 10 pages
https://doi.org/10.1155/2018/4705472