Suchismita Mishra et al JMSCR Volume 06 Issue 11 November 2018 Page 40 JMSCR Vol||06||Issue||11||Page 40-46||November 2018 Amniotic Membrane transplantation for corneal surface reconstruction Authors Suchismita Mishra, Jayashree Dora, Ramesh Chandra Mohapatra, Pragati Jain, Swati Samant* Department of Ophthalmology, IMS and SUM Hospital, Siksha O Anusandhan University, K8, Kalinga Nagar, Bhubaneswar-751003 Corresponding Author Dr Swati Samant, MS Associate Professor, Department of Ophthalmology, IMS and SUM Hospital Email: swatisamantdhar@gmail.com Abstract Objectives: To evaluate the efficacy of fresh human amniotic membrane for reconstruction in corneal surface diseases. Material and Methods: 28 eyes of 26 patients with corneal surface disorders were evaluated. Indications for AMT included bullous keratopathy (4 eyes), climatic droplet keratopathy (3 eyes), Steven Johnson syndrome (7 eyes), chemical injury (3 eyes), corneal ulcer (7 eyes), band keratopathy (2 eyes) and shield ulcers (2 eyes). Fresh amniotic membrane was used in all the cases. Result: The age range was from 18-65 years, out of which 15 were males and 11 were females. Success was noted in75% (21/28 eyes) with very few complications in an average follow up period of 6 months. The mean epithelisation time varied from 2 – 2.4 weeks. Conclusion: Fresh amniotic membrane can reduce inflammation, promote epithelisation and decrease in various corneal surface disorders. Keywords: AMT, AM, Human amniotic membrane, corneal surface disorder, SJS. Introduction Foetal membrane consists of two main layers amnion and chorion. Amniotic membrane (AM) is the innermost avascular foetal membrane derived from ectoderm. It thickness varies from 0.02 mm to 0.5 mm and it neither contains nerve or blood vessels 1, 2 . Bourne described amnion as consisting of five layers from within outward, i.e epithelium, basement membrane, compact layer, fibroblast layer and spongy layer .1 Since 1910, human amniotic membrane (HAM) has been used for many purposes. It has been used for biological dressing in acute burns 3 , skin ulcers 4 and abdominal wounds 5 . It has also been used as graft for reconstruction of otolarynx and vagina (in the absence of vagina) 6 . In ophthalmology, the first use of HAM was by de Roth in 1940 in treatment of the conjunctival defect after symblepharon caused by chemical injury and phemphigus 7 . Very little regarding amniotic membrane transplantation (AMT) was seen in ophthalmologic literature until 1995, when Kim et al used amniotic membrane for ocular surface reconstruction of severely damaged cornea www.jmscr.igmpublication.org Impact Factor (SJIF): 6.379 Index Copernicus Value: 79.54 ISSN (e)-2347-176x ISSN (p) 2455-0450 DOI: https://dx.doi.org/10.18535/jmscr/v6i11.08