BMRBiotechnology BT 38|Volume 2|Issue 1|2015 1 Review Article Methods Of Isolation Of Indigenous Saprophytic Fungi And Screening Of Their Cellulolytic Activity – A Review B. Sadhana. T. Nikhitha, S. Satya Vani & S. B. Padal Department of Botany, Andhra University, Visakhapatnam-530003, A.P, India Correspondence should be addressed to S. B. Padal Received May31, 2015; Accepted July 05, 2015; Published July 14, 2015; Copyright: © 2015 S. S. B. Padalet al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cite This Article:Sadhana,B., Nikhitha,T., Satya Vani, S.,B. Padal, S. (2015). Methods of isolation of indigenous saprophytic fungi and screening of their cellulolytic activity – A review, BMR Biotechnology 2(1). 1-4 ABSTRACT The cellulose degradation is an important commercial process. As cellulose is complex organic polymer and need to be degraded in large scale so as to process further process it into commercial products. Cellulose degradation done successfully by soil microbes’ especially saprophytic fungi. Methods of isolation of indigenous fungi, identification based on invitro characterisations, and their cellulose activity assessment are discussed in this review. KEY WORDS: indigenous saprophytic fungi, cellulolytic activity INTRODUCTION Cellulose is a most abundant organic polymer on earth ( davidB.wilson, V.Makeshkumaret.al. P.Laksminarasimha.reddy et.al.Carlo.R.carere et.al. ). It is main component of primary and secondary cell wall of the plants. Cellulose composed of thousounds of D- glucose units linked with beta 1,4 linkage(Raj kumar et al, Nazamin nayebyazdi et.al. ). As it is a complex structural element it has less direct applications (Carlo.R.care re et al). But in nature saprophytic microorganisms present in the soil employed to degrade cellulose. So many fungal and bacterial specious are reported with cellulolytic activity.But few fungal specious like.aspergillus(Nazaminnayebyazdi.et.al, Laksminarasimhareddy,V.Makeshkumaret.al .,EdwardAbayeret.al.) Pencillium(Laksminaras imhareddy et.al.) and tricoderma(Raj kumar et.al.,Laksminarasimhareddy et.al.,Nazaminnayebyazdiet.al.,R.P.Tengerd yet.al .) are generally hyper active against cellulose degradation. Cellulosomes are multi enzyme complexes. Generally present as a extracellular enzymes ( Raj kumaret.al.) attached to the surface and for efficient degradation of the plant polysaccharides especially cellulose. Fungal cellulases have somany industrial applications they widely used in textile, www.bmrjournals.com Open Access Scientific Publisher