Vol. 19, No. 2 (2020) 903-911 Revista Mexicana de Ingeniería Química Application of response surface methodology for statistical optimization of carboxymethylcellulase by Thermomyces dupontii TK-19 using submerged fermentation Aplicación de la metodología de superficie de respuesta para la optimización estadística de la fermentación sumergida de carboximetilcelulasa por Thermomyces dupontii TK-19 K. Nisar, R. Abdullah * , A. Kaleem, M. Iqtedar Department of Biotechnology Lahore College for Women University, Lahore, Pakistan. Received: September 28, 2019; Accepted: October 24, 2019 Abstract In present work, optimization of nutritional parameters for carboxymethylcellulase production was studied through experimental design. A central composite design, with 8 axial points, 16 factorial points and 6 center points were used for optimizing and predicting four independent variables such as Lactose, peptone, ammonium sulphate and Tween 80. By means of ANOVA significance of four independent variables and their possible interactions was tested with 95% of confidence level. A good agreement was obtained between predicted and experimental model. The predicted response 71.3 U/ml/min from the model showed close agreement with 72.0 U/ml/min of the experimental data which validates the effectiveness of the model while reducing the required number of experiments. Keywords: cellulase, Tween 80, CMCase, coefficient, variable. Resumen En el presente trabajo, se estudió la optimización de los parámetros nutricionales para la producción de carboximetilcelulasa a través del diseño experimental. Un diseño compuesto central, con 8 puntos axiales, 16 puntos factoriales y 6 puntos centrales se utilizaron para optimizar y predecir cuatro variables independientes como la lactosa, la peptona, el sulfato de amonio y El Tween 80. Mediante la importancia de ANOVA de cuatro variables independientes y sus posibles interacciones se probó con el 95% del nivel de confianza. Se obtuvo un buen acuerdo entre el modelo predicho y el modelo experimental. La respuesta pronosticada 71.3 U/ml/min del modelo mostró un estrecho acuerdo con 72 U/ml/min de los datos experimentales que valida la eficacia del modelo al tiempo que reduce el número requerido de experimentos. Palabras clave: celulasa, Tween 80, CMCase, coeficiente, variable. 1 Introduction Lignocellulosic material is mainly consists of cellulose, hemicellulose and lignin. These materials are considered the most abundant renewable carbon source on the earth. Cellulose is a linear chain of β-D- glucose units linked together by 1, 4-β-linkages.The lignocellulosic biomass is converted in to simple sugars with the aid of cellulases. Cellulases belong to group of hydrolytic enzymes that mediate the complete hydrolysis of cellulose. Endoglucanase (EC 3.2.1.4) randomly break the internal O-glycosidic bonds, exoglucanase (EC 3.2.1.91) acts on reducing and non-reducing ends of cellulose chain and releases end product as β-cellobiose. β-glucosidase (EC 3.2.1.21) hydrolyzes the cellobiose residues and release β-D-glucose units (Mojsov, 2016; Oliveira et al., 2019). Cellulases have various industrial applications for instance, food, textile, paper and pulp, animal feed and pharmaceutical industry, etc. Cellulases are mainly produced by different bacterial and fungal strains. Due to higher rate of enzyme production fungal cellulases are more advantageous as compared to other microorganisms. The most efficient cellulolytic fungal genera are Trichoderma, Penicillium, Humicola and Aspergillus (Gupta et al., 2015). Liquid medium is used in submerged fermentation for the growth of fungi and this type of fermentation is preferred over solid state fermentation due to better control on the process parameters including pH, aeration, temperature etc. for efficient growth of fungi consequently better production of enzyme (Mohan et al., 2013). * Corresponding author. E-mail: roheena_abdullah@yahoo.com https://doi.org/10.24275/rmiq/Bio895 issn-e: 2395-8472 Publicado por la Academia Mexicana de Investigación y Docencia en Ingeniería Química A.C. 903