202 *Corresponding Author: Fatma Sri Wahyuni (Fakultas Farmasi Universitas Andalas, Kampus Limau Manis, Kec. Pauh, Kota Padang, Sumbar 21563). email: fatmasriwahyuni@gmail.com Article History: Received: 23 Mar 2017 Accepted: 03 May 2017 Published: 21 May 2017 Available online: 30 May 2017 ABSTRACT: The sub-chronic toxicity testing of ethyl acetate fraction of fruit rind of “asam kandis” (Garcinia cowa Roxb.) to the liver and kidney function has been carried out to female white mice. A total of 18 female white mice aged 2-3 months weighing 20-30 grams are used as test animals. Animals were divided into three groups: one control group and two treatment groups. Groups were given daily ethyl acetate fraction at the doses of 500 and 1000 mg/kg orally for 60 days. Parameters observed were the activity of SGPT and liver weight ratio to observe the liver function; serum creatinine level and kidney weight ratio to determine kidney function. Data of SGPT, serum creatinine and the weight ratio of liver and kidney were analyzed by two-way ANOVA. Results show that the activity of SGPT and serum creatinine level were directly affected by the dose (p<0.05), while the organ weight ratio of liver and kidney were not affected by the dose and duration of administration (p>0.05). The study concluded that the dosage of ethyl acetate fraction of the fruit rind of G. cowa had a significant effect on the activity of SGPT and serum creatinine level of female white mice. The duration of administration did not give a significant effect on the increase of serum creatine level as well as the organ weight ratio of liver and kidney of mice. ABSTRAK: Pengujian toksisitas subkronis fraksi etil asetat kulit buah asam kandis (Garcinia cowa Roxb.) terhadap fungsi hati dan ginjal mencit putih betina telah dilakukan. Sebanyak 18 ekor mencit putih betina berusia 2-3 bulan dengan berat badan 20-30 gram digunakan sebagai hewan uji. Hewan dibagi menjadi 3 kelompok yaitu 1 kelompok kontrol dan 2 kelompoj perlakuan yang diberi fraksi etil asetat kulit buah asam kandis dengan dosis 500 dan 1000mg/kgBB sekali sehari secara oral selama 60 hari. Parameter yang diamati yaitu aktivitas SGPT dan rasio berat hati untuk fungsi hati dan aktivitas kreatinin serum dan rasio berat ginjal untuk aktivitas ginjal. Data aktivitas SGPT, kreatinin serum dan rasio berat organ hati dan ginjal dianalisis dengan ANOVA dua arah. Hasil penelitian menunjukan bahwa aktivitas SGPT, Kreatinin serum dipengaruhi secara langsung oleh dosis (p<0,05) dan untuk rasio berat hati dan ginjal tidak dipengaruhi secara langsung oleh dosis dan lama pemberian (p>0,05). Dapat disimpulkan bahwa dosis pemberian fraksi etil asetat kulit buah asam kandis memberikan pengaruh yang bermakna terhadap aktivitas SGPT dan kadar kreatinin serum mencit putih betina. Lama pemberian tidak memberikan pengaruh yang bermakna terhadap peningkatan kadar kreatinin serum mencit putih betina dan rasio berat hati dan ginjal mencit putih betina. Keywords: sub-chronic toxicity; ethyl acetat fraction; Garcinia cowa; cowa mangosteen; fruit rind. Kata Kunci: toksistas sub kronik; fraksi etil asetat; Garcinia cowa; asam kandis; kulit buah. Jurnal Sains Farmasi & Klinis (p- ISSN: 2407-7062 | e-ISSN: 2442-5435) diterbitkan oleh Ikatan Apoteker Indonesia - Sumatera Barat homepage: http://jsfkonline.org Jurnal Sains Farmasi & Klinis, 3 (2), 202-212 Uji Toksisitas Subkronis Fraksi Etil Asetat Kulit Buah Asam Kandis (Garcinia cowa Roxb.) terhadap Fungsi Hati dan Ginjal Mencit Putih Betina {Sub-chronic toxicity evaluation of ethyl acetate fraction of fruit rind of “asam kandis” ( Garcinia cowa Roxb.) against liver and kidney function of female white mice} Fatma Sri Wahyuni 1 , Intan Nedia Putri 1 , & Dessy Arisanti 2 1 Fakultas Farmasi Universitas Andalas 2 Fakultas Kedokteran Universitas Andalas