1 Plant Archives Vol. 20, Supplement 2, 2020 pp. 45-52 e-ISSN:2581-6063 (online), ISSN:0972-5210 EFFECT OF LED AND HALOGEN LIGHTS ON THE GROWTH OF TWO LOCAL MICRO ALGAE AND SOME OF THEIR MEDICALLY ACTIVE SUBSTANCES Hamida Ayal Maktof and Raid Kadhim Abed Al-Asady Biology Department, College of Education, University of Al-Qadisiyah, Iraq Email: raid.al-asady@qu.edu.iq. raid_alasady@yahoo.com iraq890iraq@gmail.com Abstract In the current study, two types of algae Spirulina platensis and Coelastrella terrestris were exposed to two different types of lighting, LED and Halogen with different light intensities, to identify the effect of lighting on the growth of algae and their production of chlorophyll-a, protein, carbohydrates and lipids, as well as investigating anti-properties. Bacterial to these algae extracts. The study showed that there are differences in the growth rates of the algae under study and exposed to the two types of lighting, as the intensity of illumination 2000 LED was the best when it recorded the highest concentration of the chlorophyll tincture for both algae while the lighting intensity was 2500 and 3000 lux for the source of halogen lighting is the best in the production of chlorophyll for algae r, Q, respectively, with respect to proteins, the highest concentration of 0.48 mg/ml was recorded by algae spirulina at the intensity of illumination of the 2000 lux LED and 0.501 mg/ml at the intensity of illumination of 3000 lux halogen. The highest concentration of carbohydrates was 0.288 mg/ml, which was recorded by the alga C. terrestris at the intensity of illumination of 3000 Lux LED, while the type of halogen lighting was the highest concentration of carbohydrates recorded by the same alga and the same intensity of illumination. The highest concentration of lipids was recorded by S. platensis, and it reached 0.196 mg/ml when exposed to the intensity of illumination of 2000 lux type LED. As for exposure to halogen lighting, the same algae gave the highest concentration of lipids and when the intensity of illumination was 3000 lux. In the current study, the inhibitory efficacy of extracts was examined Algae under study on three types of pathogenic bacteria, namely E. coli, Psedumonas aeuruginosa and Staphyllococcus aeureus. The results indicated that treatment with a concentration of 1000 mg/ml of algae extracts gave the highest inhibitory efficacy as the percentage of sensitivity to bacteria E. coli 97% and 36%, respectively. Keywords: LED, Halogen lights, local micro algae Introduction Algae are present in all ecosystems, especially in aquatic ecosystems (Barsanti and Gaulieri, 2014). They are consider as cornerstone in these ecosystems due to their ability to harvested the energy of sunlight by converting light energy to chemical energy via photosynthesis (Taiz and Zeiger, 2003). S. platensis Geitler are planktonic cyanobacteria that form massive populations in tropical and subtropical water bodies characterized by high levels of carbonate and bicarbonate and high pH (up to 11). The microalgae constituent of phoaspholipids can be altered due to changing environmental standards, growth rates, light intensity and amount by sunlight or artificial light, as lighting is a source of energy in autophototrophs as it uses light energy to convert the available nutrients and carbon dioxide into organic compounds Stored inside their cells, lighting is one of the physical factors that affect the distribution and abundance of phytoplankton, including microalgae through its direct effect on photosynthesis and growth rate, as high lighting causes oxidation of cellular components and stabilizes I necessary enzymes in the process of photosynthesis and this affects the content of algae from all active substances and compounds (Gerotti, 001). Although sunlight is the most cost-effective source of microalgae production, it suffers from defects in uncontrollable biological clock rhythms, in addition to changes in weather, season, and time during the day. It decreases with depth and this is due to the absorption of water and suspended matter, including neighborhoods. Water is like planktons, in addition to the deviation of light by water molecules, the so-called dispersion of molecules, and light scattering may occur by suspended matter also, because the blue color of the pure ocean water is caused by the dispersion of this color in the upper direction while the coastal waters have a color Green due to the presence of large quantities of suspended matter that reflect light in longer waves, and therefore artificial light may be more practical and economical in producing microalgae (Blanken, 2013) In recent years, a variety of artificial lamps, including the Halogen lamp and the second LED light-emitting lamp, have been used extensively in algae cultivation, and each is characterized by a different light intensity and wavelength that affects the biomass production of algae, that the artificial light is better in terms of Regulating the PPFD's photon flux density, light duration, wavelength and intensity of light, which can lead to an abundance of production and quality standards in algae biomass are key factors for the success of any industrial agricultural product (USDOE, 2013). The current study aimed to know the effect of two different types of lighting (LED and Halogen lamps) and different intensities used in algae culture emphasized the growth and physiology of two types of algae, one of which is the green C. terrestris and the blue green algae Spirulina platensis and the production of active substances by them. Materials and Methods Samples of the study The microalgae of this study were obtained from advanced ecology and algology lab. in College of Education of university of Al-Qadissiyah. The experiments were achieved in illuminated incubator under 25°C temperature and different light intensities of LED (LOW CRI LED 6500K) and Halogen (Halogen Lamp 3000K) sources.