IBL CONFERENCE 2017 - PROCEEDINGS Bali Medical Journal (Bali Med J) 2017, Volume 3, Number 3 (IBL Conference 2017 Special Issue): S43-S46 P-ISSN.2089-1180, E-ISSN.2302-2914 S43 Open access: www.balimedicaljournal.org and ojs.unud.ac.id/index.php/bmj CrossMark Published by DiscoverSys INTRODUCTION In order to maintain food safety, examination of hazardous substances in foodstufs must be routinely done. One of the most common elements is heavy metal, particularly lead (Pb). Lead is hazardous to human health if the amount consumed exceeds the recommended threshold. Lead, like the other heavy metals, tends to accumulate in the body of the consumer. Te lead could substitute iron in the hemoglobin and potentially caused anemia. 1 Lead poisoning could also result in various organs failure such as liver, 2 kidney, lungs, spleen, 3 and brain. 4 Beef is one of the many protein sources that are widely consumed. To obtain a healthy and safe-to- consume beef, one needs to ensure the meat quality thoroughly, and especially to watch out for the lead (Pb) content in it. Lead contamination in Bali cattle could be originated from the food, water, and even the polluted air. Te blood of the cattle herded at a landfll in Denpasar was found to be contaminated by lead with an average concentration of 6.595 ± 1.85 ppm. 5 It exceeded the maximum threshold set by SNI 7387-2009, which is 2.00 ppm. Another study had also reported that the mean of lead contamination in the blood of cattle butchered in a slaughterhouse in Nigeria was 5.65±0.70 ppm. 6 A traditional slaughterhouse is a common place of livestock slaughtering of Bali cattle that possibly came from a farm with poor animal care. Te cattle could be herded in an inappropriate and polluted location, such as a landfll. Tat is why it is necessary to conduct an examination of lead contamination in the blood of Bali cattle which were butchered in a traditional slaughterhouse. METHOD Sample Te study used blood samples from 20 cattle that were slaughtered in a traditional slaughterhouse in Denpasar, Bali. Te blood was taken using 10 ml syringe from and stored in 10 ml tubes which previously flled with anticoagulant 0.5% Ethylenediaminetetraacetic Acid (EDTA). Examination of lead concentration was conducted in the Analytical Laboratory of Udayana University. Measurement of Lead Content Level Te analysis of lead content in cattle’s the blood samples was done using AAS (Atomic Absorption Spectrophotometric) method. Each of the blood samples was taken from each cattle. Each sample was then split into two parts of 0.5ml each; one for ABSTRACT Lead contamination in the body of the cattle was thought initially to be coming from the feed and the environment in which the cattle were maintained. When a human consumes more than 2.0 ppm of lead-contaminated beef, it could cause damage to various organs, and also lower cognitive abilities. This study aims to determine the amount of heavy metal lead in the blood of Bali cattle which were slaughtered in a traditional slaughterhouse in Denpasar. Blood plasma samples from 20 Bali cattle were taken randomly and used for this study. The blood stored in 10ml tubes which were filled with 0.5% Ethylenediaminetetraacetic acid (EDTA) previously. The lead content measurement was conducted by using atomic absorption spectrophotometer (AAS) method. From the measurement result, it was obtained that the average lead content was 7.35±4.33 ppm. There was a blood sample that did not contain any lead. The lead content of this result is higher than in the cattle from Suwung Landfill Denpasar, which was 6.60±1.85 ppm, as the result of our research previously. This result shows that lead contamination does not only originate from the place in which the cattle were raised. However, other risk factors can contribute to it and thus further investigation is needed. Nonetheless, it can be concluded that the level of lead contamination in blood plasma of Bali cattle which were slaughtered in a traditional slaughterhouse is 7.35±4.33 ppm. There is the need to do a further study about the risk factors of lead contamination in the blood of Bali cattle. Keywords: lead, Bali cattle, blood plasma. Cite This Article: Berata, I.K., Kardena, I.M., Susari, N.N.W., Sudira, I.W. 2017. Lead detection in blood of the Bali cattle that were slaughtered in the traditional slaughterhouses in Denpasar, Bali. Bali Medical Journal 3(3): S43-S46. DOI:10.15562/bmj.v3i3.725 * Corresponding author: I Ketut Berata, Pathology Laboratory of Veterinary Medicine Faculty, Udayana University berata_iketut@unud.ac.id Received: 2017-07-01 Accepted: 2017-07-15 Published: 2017-07-17 1 Pathology Laboratory of Veterinary Medicine Faculty, Udayana University 2 Anatomy Laboratory of Veterinary Medicine Faculty, Udayana University 3 Farmacology & Toxicology Lab of Veterinary Medicine Faculty, Udayana University Jl. Sudirman Denpasar – Bali – Indonesia Lead detection in blood of the Bali cattle that were slaughtered in the traditional slaughterhouses in Denpasar, Bali I Ketut Berata, 1 I Made Kardena, 1 Ni Nyoman Werdi Susari, 2 I Wayan Sudira 3