© 2017. Published by The Company of Biologists Ltd. Novel ATP-synthase independent mechanism coupling mitochondrial activation to exocytosis in insulin-secreting cells Umberto De Marchi a* , Aurelie Hermant a , Jonathan Thevenet a , Yann Ratinaud b , Jaime Santo-Domingo a , Denis Barron b and Andreas Wiederkehr a a Mitochondrial Function, Nestlé Institute of Health Sciences, EPFL Innovation Park, Building G, CH-1015 Lausanne, Switzerland b Natural Bioactives and screening, Nestlé Institute of Health Sciences, EPFL Innovation Park, Building H, CH-1015 Lausanne, Switzerland * To whom correspondence should be addressed: Umberto De Marchi, Mitochondrial Function, Nestlé Institute of Health Sciences, EPFL Innovation Park, Building G, CH-1015 Lausanne, Switzerland, Tel.: +41 21 632 6122; E-mail: Umberto.DeMarchi@rd.nestle.com Keywords Mitochondria; Calcium signaling; Redox signaling; Beta-cells; Signal transduction Summary statement Secretagogues stimulating mitochondria of pancreatic beta-cells act by two different mechanisms to promote insulin secretion. The pathways are distinct in their dependence on ATP-synthase, Ca 2+ signals and redox changes. Journal of Cell Science  Advance article JCS Advance Online Article. Posted on 12 April 2017