Journal of Camel Practice and Research December 2021 / 283 SEND REPRINT REQUEST TO M.M. WAHEED email: mmwaheed@kfu.edu.sa The objective of DNA barcoding is to improve a kind’s convinced categorisation library for eukaryotes. A 650 bp DNA fragment of the cytochrome c oxidase 1 gene has been utilised effectively for kind’s level documentation in numerous animal collections (Meusnier et al, 2008). Kitano et al (2007) recognised a simple process utilising general primers for kinds documentation based on direct PCR sequencing. In humans, the estimated sizes of PCR yields of the 12S and 16S rRNAs were 215 and 244 bp, respectively. Both primer sets effectively augmented the predictable PCR products from different classes of vertebrates comprising mammals, birds, reptiles, fsh, amphibians, and the sequenced sections contained adequate nucleotide variances to categorise each animal kind (An et al, 2007). The problems faced in the separation and purification of DNA particularly from eukaryotic kinds comprise degradation of DNA owing to endonucleases, exceptionally sticky polysaccharides and other secondary metabolites which straight or indirectly inhibit with the enzymatic reactions. Different techniques for DNA extraction have been effectively useful to many eukaryotic kinds (Doyle and Doyle, 1987; Ziegenhagen et al, 1993), which were added improved to deliver DNA appropriate for numerous types of analyses (Wang and Taylor, 1993; Ziegenhagen and Scholz, 1998). The integrated primate biomaterials and material source affords vital inquiry elements to the technical public by launching, approving, preserving, and allotting RNA and DNA consequent from primate cell cultures (Lorenz et al, 2005). Common primers were utilised for the magnifcation of the mitochondrial 12S rRNA gene from genomic DNA different kinds (Gupta et al, 2008). DOI : 10.5958/2277-8934.2021.00044.8 Vol 28 No 3, p 283-290 DNA BARCODING OF MAMMALIAN SPERMATOZOA Hussein Y.A. 1,2 , Youssef M.M. 3,4 , Hereba A.M. 5,6 , Al-Shokair S.S. 1 and Waheed M.M. 1,7 1 Department of Clinical Sciences, 5 Department of Microbiology and Parasitology, College of Veterinary Medicine, 3 Department of Chemistry, College of Science, King Faisal University, P.O. Box: 400 Al-Ahsa 31982, Saudi Arabia 2 Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Alexandria University, Egypt 4 Department of Chemistry, College of Science, Mansoura University, Egypt 6 Medical Research Institute, Egypt 7 Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt ABSTRACT One of the impressive tasks of recent natural science is to improve perfect and consistent knowledge for a quick selection of semen DNA variants. This subject of investigation is of major signifcance for the recognition and documentation of kinds in several felds of exploration. Diversity of DNA grounded attitudes have been established for the documentation of entities in a numerous of taxonomic clusters. The genomic DNA was isolated from semen samples collected from different eukaryotic kinds and matched the outcomes of the results acquired in expressions of magnitude (concentration of DNA isolated and DNA gotten per ml of semen used) and superiority (260/280 relation of the gotten results). A sequences of random oligonucleotide primers were planned and utilised it with the genome DNA of entities’ kind of different eukaryotic kinds identify and estimate these kinds by using polymerase chain reaction (PCR) established assays. The DNA purifed from semen was amplifed and the migration outline of the amplifed precise long and short amplifed DNA features was measured by the beneft of agarose gel electrophoresis. The kinds specifcity of the PCR amplifcation was confrmed by the aptitude of the analyses to precisely identify and recognise kinds defnite DNA from varied sources. A critical assessment of all procedures is accessible concentrating on their biased authority, reproducibility and user kindliness. The present tendency was utilised to improve trivial measure devices with a high amount capability. Results of morphological parameters of sperms heads were tabulated, there were signifcant difference between human and animal groups (camel, ram and buck) (P<0.05). It is concluded that image J system and DNA barcode of individual spermatozoa provide perfect calculation of different semen parameters. Key words: DNA barcodes, scanning electron microscope, sperm evaluation, sperm morphometry