Journal of Faculty of Sciences - Volume No. 5, December, 2018 [1] In vitro Antimicrobial Activity and Phytochemical Screening of Ammi visnaga Against Enteric Microorganisms Abdulaziz A. Moalim 1 *, Osman Khalil 1 and Mohamud M. Ali 1 1 Department of Microbiology, Faculty of Pure & Applied Sciences, International University of Africa, P.O. Box 2469, Khartoum, Sudan * Corresponding author: Abdiaziz2004@live.com ABSTRACT This study was carried out to evaluate the antimicrobial activity and phytochemical screening of Ammi visnaga (Khella) against standard and clinical isolates from patients suffering from gastrointestinal tract infections. A total of fifty stool samples were collected from different hospitals, cultured in different media, isolated and identified. The isolated organisms were as follows: Escherichia coli (32%), Salmonella enterica (typhi) (18%), Proteus vulgaris (12%), Staphylococcus aureus (14%), Klebsiella pneumonia (10%) and Shigella dysentery (14%). The plant was extracted with two solvents (ethanol and hexane), ethanol extracts showed higher activity than hexane extracts. Effect of Ciprofloxacin and Nalidixic acid antibiotics were also tested gainst all the isolated bacteria in addition to Candida albicans (standard). Ciprofloxacin showed high activity to all the strains while Nalidixic acid gave low activity. This study proved that Ammi visnaga (100mg/ml) has inhibited all the bacteria and fungi recruited in this study, while Ciprofloxacin is the antibiotic of choice for all these tested microogranisms. Phytochemical screening of A. visnaga gave the high secondary metabolite contents e.g (Alkaloid ++, Flavonoid ++). Drug industries should recruit this