BRIEF REPORTS 601 mosome as the only human chromosome. Typing DllS2071 in 40 CEPH families allowed us to map this new marker via 2-point and multipoint linkage analysis. Linkage analysis was performed using data from the CEPH database v7 to- gether with our own data for DllS2071. The TWOPOINT and ALL options of the CRIMAP program (4) were used for pairwise and multipoint linkage analysis, respectively. The CHROMPIC option of CRIMAP was used to detect putative data errors. We observed 3 male meioses in which HRAS underwent isolated double recombinations involving the close flanking markers DllS2071 and DllS922, separated by 8 cM. In each of these cases, the existence of a string of two or more close nonrecombinant markers extending centromeric from DllS922 suggested that the typing for either p194 or for HRAS was in error, but did not allow a decision as to which of these was actually in error. Hence, data for both markers in these 3 individuals were eliminated from the da- tabase and the map was recomputed. The results of sex-spe- cific 2-point linkage analyses for DllS2071 and HRAS, the most telomeric marker on the index map of chromosome 11 (8), were as follows. The maximum likelihood recombination fractions were 0.05 (confidence limits 0.024 to 0.092) and 0.00 (confidence limits 0.00 to 0.025) in males and females, respectively, indicating that the tendency for increased re- combination in males already noted in the distal llp region (8) is maintained in the most distal interval. Indeed, all of the 7 recombinants observed between DllS2071 and HRAS and 13 of the 14 recombinants observed in the interval DllS2071-DllS922 occurred in male meioses. Multipoint analyses with a group of markers mapped to the subtelomeric region of chromosome 11 showed that the order pter-DllS2071-HRAS-DllS922-INS-cen was fa- vored over the next most likely order pter-HRAS- DllS2071-D11S922-INS-cen by odds exceeding 101°:1. Although we do not provide a direct measurement of the distance of this marker from the telomere, if the YAC is not rearranged, this distance could not exceed 140 kb. The telo- meric marker described here will be useful in genetic map- ping and positional cloning of genes mapping close to the 1 lp telomere, such as the long QT syndrome (7) and a recently described tumor suppressor gene (1). ACKNOWLEDGMENTS This work was supported by Grants HG-00022 (to M.L.) and HG00567 (to H.R.) from the National Institutes of Health. REFERENCES 1. Bepler, G., and Garcia-Blanco, M. (1994). Three tumor-suppres- sor regions on chromosome llp identified by high-resolution deletion mapping in human non-small-cell lung cancer. Proc. Natl. Acad. Sci. 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The highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. Proc. Natl. Acad. Sci. USA 89: 4913-4917. 12. Vocero-Akbani, A., Sanjurjo, H., Veile, R., Anker, R., Korte- Safarty, J., and Donis-Keller, H. (1993). Mapping of 21 human telomere YAC clones including 6 microsatellite polymorphisms identified for 2pter, 7qter, 10pter, 12pter, 13qter and 14qter. Am. J. Hum. Genet. 53: 187S. Assignment of the Human Angiotensin II Type 2 Receptor Gene (AGTR2) to Chromosome Xq22-q23 by Fluorescence in Situ Hybridization Catherine Chassagne,* Barbara G. Beatty, t and Sylvain Meloche *'1 *Centre de Recherche, H6teI-Dieu de Montreal, Montreal, Quebec, Canada H2W 1T8; and tDepartment of Pathology, The Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8 Received August 23, 1994; revisedOctober 14, 1994 Angiotensin II (AII), the biologically active effector of the renin-angiotensin system, is a major regulator of blood pres- sure and electrolyte balance and a growth factor for diverse ' To whom correspondence should be addressed. Telephone: (514) 843-2733; Fax: (514) 843-2715. GENOMICS 25, 601--603 (1995) 0888-7543/95 $6.00 Copyright © 1995 by AcademicPress, Inc. All rights of reproduction in any form reserved.