Influence of Nanoparticles on the Nervous Tissue Properties I.Reshetov * , V.Chissov * , S.Sukharev * , V.Timoshenko ** , V.Sidletsky * and P.Forsh ** * P.A.Hertzen Moscow Research Oncological Institute, 2-nd Botkinsky proezd 3, Moscow 125248, Russian Federation, hertz@portal.ru ** Physics Department, Moscow State M.V.Lomonosov University, 119999 Moscow, Russia, timoshen@phys.msu.ru ABSTRACT Interaction between nanoparticles and nervous tissue in the process of the damage healing is the object of study in the present work. The Nervus ishiadicus of rat was intersected and anastomosis performed. The electrical resistance of the nerve was measured above and below the anastomosis after place of anastamosis being underwent the action of AC field or heat. The electrical resistance of the nerve was measured above and below the anastomosis. Nanoparticles are biocompatible with respect to the nervous tissue. They decrease the pathological pulsation from the area of damage and the spread of changes in the macroscopic electrical parameters of the nervous tissue, including changes, caused by damage. Keywords: nanoparticles, biodistribution, nervous tissue 1 AIMS AND INNOVATION The surgical treatment of malignant tumors requires quite often the removal of nervous structures. This unavoidably leads to the patient invalidization, and also compromises the function of organs and muscles. The development of nanotechnologies applications to the treatment of nervous system (both central and peripheral) defeats significantly lags behind the development of applications in other areas of medicine (orthopedics, DNA- sensors, drug delivery). Among the reasons for this delay it is possible to name the following circumstances: - the difficulty of access to the divisions of central and peripheral nervous system; - extremely diverse cellular and molecular environment, in which inanosobjects should act; - the complexity of anatomical and functional connections and information streams in the nervous system. Special features of nanoparticle’s distribution and interaction with mammalian tissue were investigated earlier for muscle and epithelium tissues, tumor including. Interactions of living cells with porous silicon in-vitro were studied earlier [1,2]. Interaction between silicon nanoparticles and nervous tissue in the process of the damage healing is the object of study in the present work. 2 MATERIALS AND MODEL 2.1 Biological Model In this study we used the rat femur model. For the purpose of this investigation 40 rats (250–300 g ) were used. Three groups of rats each comprises 10 – 15 animals were formed. The first group (control) – no nanoparticles used. The second one was subjected to action of AC field (Figure 1) after nanoparticles injection into anastomosis area. Figure 1: Stimulation of anastomosis area by AC field. The animals of third group underwent the action of heat after anastomosis performance. An anesthetic solution containing phentanili and droperidoli was injected intraperitoneally. Surgery was performed on the front of the lower limb, unilaterally under sterile conditions. The Nervus ishiadicus of rat was intersected and anastomosis performed. Rats were sacrificed at 1 – 49 days after operation. Surgical procedures were conducted in compliance with ethical principles for animal research, as approved by NSTI-Nanotech 2008, www.nsti.org, ISBN 978-1-4200-8504-4 Vol. 2 161