Research Article
The Estimation of Blood Paramagnetic Center Changes during
Burns Management with Biodegradable Propolis-
Nanofiber Dressing
Pawel Olczyk ,
1
Katarzyna Komosinska-Vassev ,
2
Ryszard Krzyminiewski,
3
Janusz Kasperczyk,
4,5
Pawel Ramos ,
6
Bernadeta Dobosz,
3
Olgierd Batoryna,
1
Jerzy Stojko ,
7
Mateusz Stojko ,
4,5
Diana Ivanova ,
8
Krystyna Olczyk ,
2
and Barbara Pilawa
6
1
Department of Community Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice,
Sosnowiec, Poland
2
Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University
of Silesia in Katowice, Sosnowiec, Poland
3
Medical Physics Division, Faculty of Physics, Adam Mickiewicz University, Poznan, Poland
4
Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze, Poland
5
Department of Biopharmacy, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of
Silesia in Katowice, Sosnowiec, Poland
6
Department of Biophysics, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice,
Sosnowiec, Poland
7
Center of Experimental Medicine, Medics 4, Faculty of Medicine in Katowice, Medical University of Silesia in Katowice,
Katowice, Poland
8
Department of Biochemistry, Molecular Medicine and Nutrigenomics, The Faculty of Pharmacy, Medical University of Varna,
Varna, Bulgaria
Correspondence should be addressed to Pawel Olczyk; polczyk@sum.edu.pl
Received 1 July 2019; Revised 7 June 2020; Accepted 9 June 2020; Published 29 June 2020
Guest Editor: Reggiani Vilela Gonçalves
Copyright © 2020 Pawel Olczyk et al. This is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The evolution of the paramagnetic center system in blood during the healing of skin burn wounds dressed with a biodegradable
apitherapeutic nanofiber dressing was examined. The aim of this study was to determine the changes in paramagnetic centers in
blood during the influence of apitherapeutic nanofiber dressings on the healing process. The blood samples were tested before
burn infliction (day 0) and, respectively, on the 10
th
and 21
st
days of the experiment. Paramagnetic centers in the blood of the
pig used as the model animal were examined with an X-band (9.3 GHz) electron paramagnetic resonance spectroscopy. The
EPR spectra were measured with Bruker spectrometer at 230 K with a modulation frequency of 100 kHz. The EPR lines of the
high spin Fe
3+
in methemoglobin, high spin Fe
3+
in transferrin, Cu
2+
in ceruloplasmin, and free radicals were observed in the
multicomponent spectra of blood. For the application of the apitherapeutic nanofiber dressing, the amplitudes of the EPR
signals of Fe
3+
in methemoglobin were similar up to 10 days. For the experiment with the apitherapeutic formulation, the
heights of EPR signals of Fe
3+
in transferrin were lower after 10 days and 21 days of therapy, compared to day 0. For the
application of the apitherapeutic formulation the signals of Cu
2+
in ceruloplasmin and free radicals, strongly decreased after 10
days of therapy, and after 21 days it increased to the initial values characteristic for day 0. The apitherapeutic formulation
caused that after 21 days the EPR spectrum of Cu
2+
in ceruloplasmin and free radicals was considerably high. The
apitherapeutic formulation interaction after 10 days and after 21 days of therapy resulted in the low EPR lines of Fe
3+
in
methemoglobin. EPR spectra of blood may be useful for presentation of the changes in its paramagnetic centers during the
healing process of the burn wounds.
Hindawi
Oxidative Medicine and Cellular Longevity
Volume 2020, Article ID 3675603, 9 pages
https://doi.org/10.1155/2020/3675603