Sawsan Ahmed et al./ Elixir Applied Science 161 (2021) 55842-55844 55842 Applied Science Introduction Most of the information about our physical surrounding comes to us through our senses of hearing and sight, sound and light are waves. A wave is carries energy from one place to another without a transfer of mass. Sound is a mechanical wave produce by vibrating bodies, when (source)is set in to vibrational motion the surrounding air molecules are disturbed and are forced to follow the motion of the vibrating body. the vibrating molecules in turn transfer their motion to adjacent molecules causing the vibrational disturbance to propagate away from the frog when the air vibration reach the ear (receives)they cause the eardrum to vibrate, this produces nerve impulses that are interpreted by the brain [1,2]. The sound is transmitting according to a material medium between the source and the receiver and the distant. The sound transfer by compression and rarefaction in the medium initially by the vibrating sound source ,intensity of sound are determined by the magnitude of compression and rarefactions in the medium and frequency is measured in cycles per second and designated by (hertz )(1 Hz = 1 cycle per second ).The distance between the nearest equal points on the sound wave is called the wave length (λ), we can circulate the speed of sound wave (ν) by know the material that propagate the sound, in air at 20◦C the speed of sound is about 3.3 X 10⁴ cm \sec and in water it is about 1.4 X 10⁵ cm \sec , in general the relationship between frequency . There are four important phenomena which described a sound waves reflection refraction, interference, and diffraction [3,4]. Material & Method Apparatus -Laser Source Output power <200 mV, Wave length 405 nm - USB 2000 Spectrometer Experiment Setup The three leaves plant samples were placed as shown respectively at the USB2000 spectrometer. The samples using USB2000 device to take the readings Method The three plants leaves were exposed to laser source, then the USB 2000 spectrometer were used as shown above. The USB2000 Spectrometer connects to a notebook or desktop PC via USB port or serial port. When connected to the USB port of a PC, the USB2000 draws power from the host PC, eliminating the need for an external power supply. Then readings were taken Spectroscopic techniques discipline that uses mathematics, statistics and formal logic (a) to design or select optimal experimental procedures; (b) to provide maximum relevant chemical information by analyzing chemical data; and (c) to obtain knowledge about chemical systems. Spectroscopic techniques excel by their possibility to gain rapid and accurate information from the high- resolution spectra of solid and liquid samples, The Spectroscopic techniques region of the electromagnetic Tele: E-mail address: sawsan.ahmed110@gmail.com © 2021 Elixir All rights reserved Elixir Applied Science 161 (2021) 55842-55844 ARTICLE INFO Article history: Received: 14 November 2021; Received in revised form: 6 December 2021; Accepted: 16 December 2021; Keywords Pandanus Amaryalifolious, Spectroscopic Techniques. Determination of the Effect of Sound on Pandanus amaryalifolious Leaves Extracts Using Spectroscopic Techniques Sawsan Ahmed, Elhouri Ahmed and Marwa Taha ELata Mohamed University of Bahri – College of Applied & Industrial Sciences Department of Physics – Khartoum – SudanPh.D. Student - Khartoum, Sudan. ABSTRACT In this work two samples of a (Pandanus amaryalifolious) leaves extracts, were exposed to sound waves length of (528 nm and 741 nm) while the third sample of the leaves was left without exposure to any sound. The effect of different wave lengths absorption shows a dramatic difference at the organic compounds and sulfur compounds of each sample, these differences were detected using spectroscopic techniques. © 2021 Elixir All rights reserved. Available online at www.elixirpublishers.com (Elixir International Journal)