A revision of Trigonella sect. Ellipticae (Fabaceae) in Iran
M. Ranjbar, R. Karamian, Z. Hajmoradi and M. R. Joharchi
M. Ranjbar (ranjbar@basu.ac.ir), R. Karamian and Z. Hajmoradi, Dept of Biology, Herbarium division, Bu-Ali Sina Univ., PO Box 65175/4161,
Hamedan, Iran. – M. R. Joharchi, Herbarium of Ferdowsi, Univ. of Mashhad, Iran.
In this paper a key, descriptions and new results are provided for Trigonella sect. Ellipticae occuring within the area covered
by the flora of Iran. Trigonella sect. Ellipticae comprises perennial herbs, becoming woody at the base, with standard as long
as keel and pods glabrous or rarely covered with gray dense and appressed hairs. Trigonella lasiocarpa Ranjbar & Hajmoradi,
T. binaloudensis Ranjbar & Karamian, T. stipitata Ranjbar & Joharchi and T. torbatejamensis Ranjbar are described and
illustrated as new species based on habit and pollen morphology. Te relationships between the new species and their
closest relatives are discussed. Te pollen morphology of 26 specimens belonging to 11 species was studied with light and
electron microscopy and discussed in the context of existing hypotheses on the systematic relationships within T. sect.
Ellipticae. In addition, T. heratensis Rech. f. and T. adscendens Aphan. & Gontsch. are reported as new records for Iran.
Te tribe Trifolieae in the family Fabaceae includes 6 genera:
Trigonella, Medicago, Trifolium, Melilotus, Parochetus and
Ononis (Heyn 1981, Lock and Simpson 1991, Mabberely
1997). Te tribe, as proposed by Berchtold and Presl (1820),
is characterized by having trifoliate leaves and stipules adnate
to the petioles. Following the progression of modern tools
in taxonomy, numerous taxonomic changes and rearrange-
ments have been proposed in these genera. Trigonella L. is
one of the largest genera in the tribe Trifolieae and comprises
about 135 species widely distributed in dry regions around
the east Mediterranean, west Asia, south Europe, north
and south Africa, with only one species growing in south
Australia (Townsend and Guest 1974, Polhill and Raven
1981, Kawashty et al. 1998). Trigonella species are annual or
perennial herbs with pinnately trifoliate leaves, often emitting
an odour, and like other grain legumes, are important in food
and medicine production (Chopra et al. 1956, Girardon
et al. 1989, Balodi and Rao 1991, Bhatti et al. 1996, Dangi
et al. 2004). In ‘Flora Iranica’, the genus was represented
by 58 annual and perennial species referred to 12 sections
(Rechinger 1984). Many of the perennials are endemic to
Iran and belong to T. sect. Ellipticae. Te leaves, stems and
pods in most of the species of T. sect. Ellipticae are glabrous
or sparsely hairy, the hairs are not much variable in length
and the flowers of all species have a yellow or violet colour.
Trigonella lasiocarpa is the only member of the section that
is densely covered with white hairs. Te hairs (up to 0.5 mm
long) are found on the leaves and calyx surfaces. Trigonella
binaloudensis is the only member of the section in Iran which
has exclusively bi-coloured flowers.
Studies on the pollen grains of the family Fabaceae have
dealt mainly with the description of the pollen of certain
genera or sometimes tribes (e.g. Clarke and Kupicha 1976,
Ferguson and Skvarla 1981, Ferguson 1990, Ferguson and
Stirton 1993, Diez and Ferguson 1994, Hughes 1997).
Investigations on pollen morphology (Small et al. 1981) and
pollen–ovule patterns (Small 1986) performed in order
to resolve relationships within and between the genera
Medicago and Trigonella have failed to find rigorous diagnos-
tic characters (Bena 2001).
In this paper four new species, T. lasiocarpa Ranjbar &
Hajmoradi, T. binaloudensis Ranjbar & Karamian, T. stipitata
Ranjbar & Joharchi and T. torbatejamensis Ranjbar, are
described and two new records, T. heratensis Rech. f. and
T. adscendens Aphan. & Gontsch., are reported from Iran
and compared with their closest relatives using morphology
and pollen micromorphology based on recent botanical col-
lections by the staff of the herbarium of Ferdowsi, Univ. of
Mashhad (FUMH). Te new species have been compared
with known species during the first and second author’s vis-
its to the herbaria W and WU in 2005 and 2007.
Material and methods
Pollen morphology
For light microscopy (LM), pollen samples were obtained
from herbarium specimens of the Bu-Ali Sina Univ. her-
barium or directly collected in the field, prepared using the
standard method described by Erdtman (1960), and docu-
mented by voucher specimens (Table 1). Ten, they were
mounted on unstained glycerin jelly and observed with a
Nikon type-2 microscope. Measurements were based on
Nordic Journal of Botany 30: 17–35, 2012
doi: 10.1111/j.1756-1051.2009.00171.x,
© 2012 Te Authors. Nordic Journal of Botany © 2012 Nordic Society Oikos
Subject Editor: Arne Strid. Accepted 27 April 2009
17