~ 410 ~ Journal of Pharmacognosy and Phytochemistry 2018; 7(1): 410-413 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(1): 410-413 Received: 15-11-2017 Accepted: 17-12-2017 Hanafi Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Septilina Melati Sirait Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Endri Purwanti PT. Smart, Tbk, Bogor, Indonesia, Jl. Raya Cijayanti, Kelurahan Cijayanti, Babakan Madang, Bogor, Indonesia Candra Irawan Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Henny Rochaeni Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Tita Rosita Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Juni Aldhani Purba Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Correspondence Hanafi Polytechnic of AKA Bogor, Bogor, Indonesia, Jl. Pangeran Sogiri No. 283, Tanah Baru, Bogor, Indonesia Comparison of Abscisic Acid hormone on the leaves of palm oil seeds with watered and not watered Hanafi, Septilina Melati Sirait, Endri Purwanti, Candra Irawan, Henny Rochaeni, Tita Rosita and Juni Aldhani Purba Abstract The dryness had the effect to increase the level of abscisic acid in oil palm. The increasing level of abscisic acid in the leaves will induce and regulate stomatal closure so the dryness can be prevented. Dryness can be prevented by watered regularly. The average abscission rate was 2.2361 (μg/g) and watered leaves was 2,3832 (μg/g). Significance different test was at 95% confidence interval and obtained Pvalue equal to 0,005 and value α = 0,05. The average abscisic of oil palm which not watered was higher than the average abscission acid level with watered. Keywords: abscisic acid, hormone, oil palm, watered 1. Introduction Abscisic acid was one of the growth hormone which related to environmental stress conditions. An increasing in the concentration of abscisic acid in plant roots was a chemical signal that will be transported to the leaves when the plant has lack water from the soil. When dryness stress, the increasing of the synthesis abscisic acid in plant roots as a response to deficit groundwater will happened. This increasing in abscisic acid was related to the status of water in plants [1] . The next process of abscisic acid will be transported from root to leaves through xylem. Besides in roots, the plant also synthesizes abscisic acid in the leaves in case there is an increasing abscisic acid level. The plant growth and productivity are strongly influenced by natural condition in the form of various biotic and abiotic factors. The dryness is the most important abiotic factors in agriculture. Prolonged dryness stress can inhibit the growth of oil palm which affect the increasing of abscisic acid hormone. The increasing level of abscisic acid in the leaves will induce and regulate stomatal closure, whereas increased levels of abscisic acid in the roots increase hydraulic conductivity that increases water retrieval and transport. Dryness can be prevented by watering regularly. To determine the influence of watering the oil palm plant, then the comparison of abscisic acid in oil palm’s seed which watered and not watered has been done [2] . This research was aimed to determine the effect of dryness against abscisic acid in oil palm seed by comparing the content of the sample with watered and with not watered. The result then processed statistically using minitab 17 software. 2. Material and methods 2.1 Plant Material Seed of oil palms aged 10-11 months. 2.2 Sample Preparation Oil palm leaves were put into plastic bag and stored temporarily in cool boxes and then cleaned of dirt and dust that was attached and then cut into small pieces and mashed with liquid nitrogen. The Conditioning UPLC Tool The mobile phase used were aquadest with pH 4, methanol, acetonitrile, and aquadest with flow rate 0.2 mL/ minute, 3 minutes retention time, 1 μL injection volume, and at 208 nm wavelength. Qualitative Test The sample was injected 1 μL into the mobile phase and will enter the column. And then