Research Article
Identification of Potential Metabolic Markers of Hypertension in
Chinese Children
Jiahong Sun ,
1
Min Zhao ,
2
Liu Yang ,
1
Xue Liu ,
1
Lucia Pacifico ,
3
Claudio Chiesa ,
4
andBoXi
1
1
Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan,
Shandong, China
2
Department of Nutrition and Food Hygiene, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan,
Shandong, China
3
Department of Maternal and Child Health, Sapienza University of Rome, Rome, Italy
4
Institute of Translational Pharmacology, National Research Council, Rome, Italy
Correspondence should be addressed to Bo Xi; xibo2007@126.com
Received 20 December 2020; Revised 14 July 2021; Accepted 16 August 2021; Published 25 August 2021
Academic Editor: Yimin Zhu
Copyright © 2021 Jiahong Sun et al. is is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background. Studies in adults have shown that several metabolites across multiple pathways are strongly associated with hy-
pertension. However, as yet, to our knowledge, no study has investigated such association in childhood. We, therefore, compared
the serum metabolite profile of children with normal and elevated blood pressure (BP) to identify potential metabolic markers and
pathways that could be useful for the assessment of pediatric hypertension. Methods. e study included 26 hypertensive children
(age range, 6–11 years) and 26 age- and sex-matched ones with normal BP, who were recruited from the baseline survey of the
Huantai Childhood Cardiovascular Health Cohort Study. Ultrahigh-performance liquid chromatography-quadrupole time-of-
flight-mass spectrometry was performed to assess the serum metabolite profile. Logistic regression analysis was used to select
significant metabolites associated with hypertension after adjustment for body mass index, waist circumference, and lipid profile.
Kyoto Encyclopedia of Genes and Genomes (KEGG) and MetaboAnalyst were utilized to search for the potential pathways of
metabolites. Results. A total of 45 and 34 metabolites were preliminarily screened in positive and negative modes, respectively
(variable importance in the projection (VIP) > 1.0 and P < 0.05). After adjustment for the false discovery rate, 7 and 1 differential
metabolites in the positive and negative modes, respectively, remained significant (VIP > 1.0 and q < 0.05). ese metabolites were
mainly involved in amino acid metabolism and glycerophospholipid metabolism. Among these, two significant metabolites
including ethanolamine and 2-methyl-3-hydroxy-5-formylpyridine-4-carboxylate displayed an area under the curve value of
0.820 (95% confidence interval, 0.688–0.951), with a sensitivity of 0.846 and a specificity of 0.769. Conclusion. e untargeted
metabolomics approach effectively identified the differential serum metabolite profile in children with and without hypertension.
Notably, two metabolites including ethanolamine and 2-methyl-3-hydroxy-5-formylpyridine-4-carboxylate exhibited a good
discriminative ability to identify children with hypertension, providing new insights into potential mechanisms of
pediatric hypertension.
1.Introduction
Hypertension ranks as the predominant risk factor for
cardiovascular disease (CVD) and related mortality
worldwide [1, 2]. In addition to its high prevalence in adults,
hypertension has recently shown a rapid rise in prevalence
among children and adolescents, trending to manifest at an
earlier age. Based on 179,561 children and adolescents aged
3–20 years, a recent meta-analysis showed that the global
prevalence of pediatric hypertension, defined as having an
elevated blood pressure (BP) at all three different visits, was
about 3.0% [3]. Compelling evidence has shown a positive
association between pediatric hypertension and target organ
damage, such as microalbuminuria, left ventricular
Hindawi
International Journal of Hypertension
Volume 2021, Article ID 6691734, 8 pages
https://doi.org/10.1155/2021/6691734