Journal of Advanced Biomedical & Pathobiology Research Vol.5 No.1, March 2015, 1-8 1 Identification of immunogenic soluble protein of Pasteurella multocida Noor Masyitah Jumahat* 1 , Zaini Mohd Zain 1 , Jamal Hussaini 1 1 Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh Selangor, Malaysia. noormasyitah@yahoo.com ISSN: 2231-9123 ABSTRACT Background and objective: Pasteurella multocida is a gram negative bacterium, causative agent of economically significant diseases in domestic animals. Treatments of infected animals are complex and ineffectual due to increasing antibiotic resistance strains. Moreover, the efficacy and safety of available vaccines are limited. The objective of this study is to determine immunogenic soluble protein of Pasteurella multocida capable of protecting animals from infection with acute pasteurellosis. Methods: Bacterial culture strain was cultured in brain heart infusion (BHI) medium. Soluble proteins were extracted and separated electrophoretically using 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunogenic soluble proteins were detected by western blotting using anti-Pasteurella serum raised in rabbit against whole cell antigens of Pasteurella multocida and anti-rabbit antibody. Result: The separation of soluble proteins showed various molecular weights on the gel, ranging from 10kDA to 170kDA. According to the western blot analysis, the most intense band detected was of approximately 28kDA. Conclusion: The detected protein band will be identified and immunogenicity study of soluble protein will be carried out in response to immunogenic roles against pasteurollosis and potential role in vaccine development. Keywords: Pasteurella multocida; Pasteurollosis; Protein; Immunogenic protein. 1. Introduction Pasteurella multocida (P.multocida) is a gram negative bacterium which is causative agent of Haemorrhagic septicaemia and pathogenic in domestic animals. The infection is well known over a century and termed as pasteurellosis. The range of animals infected by P. multocida is very wide with different manifestations. The bacteria have a broad range of host but the uncharacteristic property is still not understood. P. multocida has an almost unlimited host range in mammals and birds (Dziva et al, 2008). P. multocida have five types of capsular serotypes which range from type A, B, D, E and F and each types of capsular type produce different kind of disease among animals. P.multocida type A is known for chloral in fowl and pneumonia in cattle , sheep and pig meanwhile capsular type D produces atrophic rhinitis in pig and snuffles in rabbits (Ranjan et al, 2011). Capsular type F is predominantly associated with poultry disease, particularly turkeys, although it has occasionally been reported in ruminants. In cattle, capsular serotypes B and E are associated with hemorrhagic septicaemia in Asian (serotype B) and African (serotype B) countries. Under the Carter- Heddleston