International Journal of Advanced Research in Botany (IJARB) Volume 5, Issue 1, 2019, PP 8-13 ISSN No. (Online) 2455-4316 DOI: http://dx.doi.org/10.20431/2455-4316.0501002 www.arcjournals.org International Journal of Advanced Research in Botany (IJARB) Page | 8 Phytochemical Profiling and Bioactivities of Pholidota pallida Lindl Seema Akter, Mohammed Kamrul Huda, Minhajur Rahman*, Mohammed Mozammel Hoque, Tarina Akter Eva Department of Botany, University of Chittagong, Chittagong-433, Bangladesh 1. INTRODUCTION Orchidaceae family has huge therapeutic potential and such plants are utilized as therapeutics since ancient times [1] . Orchids have been also used in traditional system of medicine for various ailments [2] . The tubers and pseudobulbs of Orchis latifolia, Orchis mascula, Cymbidium aloifolium, Zeuxine strateumatica, and some species of Dendrobium, Eulophia and Habenaria are restorative and prescript for various diseases [3] . Vanda is a well-known anti-inflammatory plant [4-5] and anti- proliferative against various cancer types [6] . Contrariwise, biological studies also apprise that, orchids harbor phytochemicals physic for fatal diseases [7] . Thence, orchid derived compounds have been receiving tremendous attention as therapeutics [8] . Bangladesh is also a reservoir of 178 orchid species of which 26 were reported to have ethnomedicinal uses [9] . Pholidota pallida is an indigenous epiphytic orchid of Bangladesh with the pseudobulbs occurring in the plains and as well as in elevation [10] . It is a traditional haemostate and healing of insomnia, fractured bones, nasal, abdominal and rheumatic pains [11-18] . But, none of above mentioned therapeutic properties of this orchid has yet been studied regarding its pharmacological value or active chemical constituents. A study on the pharmaceutical value of this medicinal orchid would be the step to explore a possible sustainable use of it. Considering all these factors, Pholidota pallida was picked for exploring its phytochemicals and bioactivities through standard methods. 2. MATERIALS AND METHODS Leaf, stem and root extracts of Pholidota pallida Lindl. were used in the present work. For extraction, naturally grown plant sample collected from Teknaf, Cox’s bazar was cleaned, chopped, air dried at room temperature and finally ground into coarse powder. It was then ground into coarse powder by using grinding machine and stored in airtight container for further investigation. Mixing of one part with another was carefully avoided. Extract was filtered through Whatman No.1 filter paper and evaporated to dryness under vacuum below 50 ºC to get the blackish extract. Then the concentrated crude extract was separated into four different solvent systems (Methanol, n-Hexane, Butanol-1, Abstract: In the present work, Pholidota pallida was explored for its phytochemicals along with its bioactivities. The qualitative screening of plant extract confirmed the presence of alkaloids, flavonoids, terpenoids, tannins, steroids and traces of quinone and coumarin in it. Four fractions viz. Methanol (FM), n- Hexane (FH), Butanol-1(BW) and Dichloromethane (FD) of methanolic crude of its leaf, stem and root were investigated for free radical scavenging, anti-inflammatory and anti-microbial activities. The responses were very unique for different dose and plant parts. The highest scavenging activity exhibited by the BW of bulb was 98.94% at 50 μg/ml dose. The highest anti-inflammatory activity was observed in FD of the plant leaf which was 79.16%. The inhibitory effect of different parts of the plant against four pathogenic bacteria was varied i.e. for S. aureus (FD of root; 17.5 mm), for B. subtilis (FD of bulb; 16.5 mm), for S. typhii (BW of root; 12mm), for B. cereus (BW of root; 9.5 mm) and root was found most effective part. The investigation indicates that, Pholidota pallida is a medicative plant having bountiful phytochemicals with antioxidant, anti- inflammatory and antimicrobial actions. Keywords: Phytochemicals, anti-microbial, anti-oxidant, anti-inflammatory. *Corresponding Author: Minhajur Rahman, Department of Botany, University of Chittagong, Chittagong-433, Bangladesh