A division of Canadian Science Publishing a not-for-profit publisher Advanced Search Browse the journal List of issues e-First articles Just-IN articles Current issue Special issues Most read articles Most cited articles Sample issue Author index For authors About the journal Open Access Benefits and services Instructions to authors Submit a manuscript Permission forms Reprints & permissions to reuse content Article « Previous TOC Next » Full Text PDF (640 K) PDF-Plus (696 K) Citing articles Effects of severe caloric restriction from birth on the hearts of adult rats Dirceu Sousa Melo, a Tania Regina Riul, b Elizabeth Adriana Esteves, b Patrícia Lanza Moraes, e Fernanda Oliveira Ferreira, d Mariana Gavioli, f Márcia Netto Magalhães Alves, f Pedro William Machado Almeida, f Silvia Guatimosim, f Anderson José Ferreira, e Marco Fabricio Dias Peixoto c a Department of Biological Sciences, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil. b Department of Nutrition, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil. c Department of Physical Education, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil. d Department of Basic Sciences, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil. e Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. f Department Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Corresponding author: Marco Fabrício Dias Peixoto (e-mail: marcofabriufvjm@gmail.com). Published on the web 11 February 2013. Received October 12, 2012. Accepted January 8, 2013. Applied Physiology, Nutrition, and Metabolism, 2013, 38(8): 879-885, 10.1139/apnm-2012-0387 ABSTRACT There has been increasing evidence suggesting that a severe caloric restriction (SCR) (above 40%) has beneficial effects on the hearts of rats. However, most of the reports have focused on the effects of SCR that started in adulthood. We investigated the consequences of SCR on the hearts of rats subjected to SCR since birth (CR50). From birth to the age of 3 months, CR50 rats were fed 50% of the food that the ad libitum group (AL) was fed. Thereafter, a maximal aerobic test was performed to indirectly evaluate global cardiovascular function. Indices of contractility (+dT/dt) and relaxation (−dT/dt) were analyzed in isolated heart preparation, and cardiomyocyte diameter, number, density, and myocardium collagen content were obtained through histologic analysis. Ventricular myocytes were isolated, using standard methods to evaluate phosphorylated AKT levels, and Ca 2+ handling was evaluated with a combination of Western blot analysis, intracellular Ca 2+ imaging, and confocal microscopy. CR50 rats exhibited increased aerobic performance and cardiac function, as shown by the increase in ±dT/dt. Despite the smaller cardiomyocyte diameter, CR50 rats had an increased heart−body weight ratio, increased cardiomyocyte density and number, and similar levels of myocardium collagen content, compared with AL rats. AKT was hyperphosphorylated in cardiomyocytes from CR50 rats, and there were no significant differences in Ca 2+ transient and SERCA2 levels in cardiomyocytes between CR50 and AL rats. Collectively, these observations reveal the beneficial effects of a 50% caloric restriction on the hearts of adult rats restricted since birth, which might involve cardiomyocyte AKT signaling. Keywords: severe caloric restriction, heart function, cardiomyocytes, AKT signaling, Ca 2+ handling References Ahmet I, Tae HJ, Cabo R, Lakatta EG, Talan MI. 2011. Effects of calorie restriction on cardioprotection and cardiovascular health. J. Mol. Cell. Cardiol. 51: 263-271 CrossRef, Medline. Almeida PW, Gomes-Filho A, Ferreira AJ, Rodrigues CE, Dias-Peixoto M, Russo RC, et al. 2009. Swim training suppresses tumor growth in mice. J. Appl. Physiol. 107(1): 261-265 CrossRef, Medline. Bers DM. 2002. Cardiac excitation–contraction coupling. Nature 415(6868): 198-205 CrossRef, Medline, ISI. Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 72: 248-254 CrossRef, Medline, ISI. Broderick TL, Belke T, Driedzic WR. 2002. Effects of chronic caloric restriction on mitochondrial respiration in the ischemic reperfused rat heart. Mol. Cell. Biochem. 233: 119-125 CrossRef, Medline, ISI. Cicogna AC, Padovani RC, Okoshi K, Matsubara LS, Aragon FF, Okoshi MP. 2001. The influence of temporal food restriction on themperformance of isolated cardiac muscle. Nutr. Res. 21: 639-648 CrossRef. Tweet 2 Article Tools Add to Favorites Download Citation Email a Colleague Reprints & Permissions Citation Alerts Download Adobe Reader for PDFs Journal Tools Instructions to authors Get an email alert for the latest issue Check out the journal's featured content Follow the Journal Subscribe Now or click here for more information Home > Journals > Applied Physiology, Nutrition, and Metabolism > List of Issues > Volume 38, Number 8, August 2013 > Effects of severe caloric restriction from birth on the hearts of adul... Subscriber access provided by UNIFESP UNIVERSIDADE FEDERAL DE SAO PAULO Login Register Shibboleth Mobile Cart What we're blogging about APNM Undergraduate Research Excellence Award Winner Profile: Rachel Strohm Posted: Sep 7, 2015 By Rachel Strohm Canadian Science Publishing sponsors the Applied Physiology, Nutrition, and Metabolism Undergraduate Research Excellence Awards, which are awarded… Home CSP Journals Books Compilations Open Access Authors Librarians Societies About the Press Contact Français Effects of severe caloric restriction from birth on the hearts of adult rats... http://www.nrcresearchpress.com/doi/abs/10.1139/apnm-2012-0387#.... 1 de 2 17/09/2015 09:10