Int.J.Curr.Microbiol.App.Sci (2017) 6(4): 1815-1821 1815 Original Research Article https://doi.org/10.20546/ijcmas.2017.604.217 Serological Survey of Brucellosis in Camel of Gujarat H.C. Chauhan 1 , Kirit B. Patel 1 *, S.I. Patel 1 , B.K. Patel 1 , B.S. Chandel 1 , A.G. Bhagat 1 , M.V. Patel 1 , S.S. Patel, A.C. Patel 1 , M.D. Shrimali 1 and Rajeshwari Shome 2 1 Department of Animal Biotechnology and Microbiology, COVSc & AH, SDAU, Sardarkrushinagar-385006, Gujarat, India 2 NIVEDI (ICAR), Bengaluru, India *Corresponding author ABSTRACT Introduction Brucellosis is caused by various species of the genus Brucella, which is the second most widely spread zoonosis worldwide (Dawood, 2008). It is one of the infectious diseases, which poses major constraint for animal production. The disease is an important public health problem in many parts of the world including India (Pal, 2007; Hadush and Pal, 2013). Brucellosis is burning problem in Gujarat, where cases of human brucellosis are reported along with high sero-prevalence in animals. Brucella can affect almost all domestic species and cross transmission can occur between cattle, sheep, goat, camel and other species (Ghanem et al., 2009). Brucellosis was reported in camels as early as 1931 (Solonitsuin, 1949); since then, the disease has been reported from all camel-keeping countries (Gwida et al., 2012). Camels are frequently infected with Brucella organisms, especially when they are in contact with infected large and small ruminants (FAO/WHO, 1986; Radwan et al., 1992). Brucellosis is characterized by abortion and birth of non-visible offspring in females; orchitis and epididymitis in males (Radostits et al., 2007). The disease is also associated with infertility and prolonged calving intervals and has considerable impact on camel production. It may also causes, chronic inflammation of joints, tendon sheath and synovial bursa especially at the carpus (Abbas International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 4 (2017) pp. 1815-1821 Journal homepage: http://www.ijcmas.com Brucellosis is a zoonotic bacterial disease of ruminants and also reported from camel. Camels are one of the most important sources of livelihood for the nomadic population of Gujarat. There are limited published information regarding the sero-epidemiology of camel brucellosis in Gujarat. Therefore, present study was aim to determine the brucella specific antibodies in camel from three camel rearing districts of Gujarat using RBPT, i-ELISA and MRT. On screening of 352 serum samples, 41(11.64%) and 16 (4.54%) samples found positive by RBPT and i-ELISA, respectively. 8 (15.38%) samples of milk found positive from total 52 samples by MRT. It was also revealed from the study that the female camel have high seroprevalence of brucellosis than male. Prevalence of brucellosis among the camels of various districts of north Gujarat indicates that the disease is endemic and may act as the source of infection for other susceptible animals including human beings. Keywords Brucellosis, Camel, RBPT, MRT, i-ELISA, PCR. Accepted: 15 March 2017 Available Online: 10 April 2017 Article Info