NUSANTARA BIOSCIENCE ISSN: 2087-3948 Vol. 9, No. 3, pp. 268-274 E-ISSN: 2087-3956 August 2017 DOI: 10.13057/nusbiosci/n090305 The appearance of rabbit skin tissue (Oryctolagus cuniculus) after supplementation of Aloe vera and Spirulina fusiformis YASMI PURNAMASARI KUNTANA 1,♥ , HUSMY YURMIATI 2 , ASRI PENI WULANDARI 1 , FARIDA SYAFITRI 3 , RUHYAT PARTASASMITA 1,♥♥ 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. Jl. Ir. Soekarno Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia. Tel. +62-22-7796412 line 104, Fax. +62-22-7794545, email: y.purnamasari@unpad.ac.id; ruhyat.partasasmita@unpad.ac.id 2 Departmenf of Animal husbandry production. Faculty of Animal Husbandry, Universitas Padjadjaran. Jl. Ir. Soekarno Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia 3 Program of Biology, Faculty of Mathematic and Natural Sciences, Universitas Padjadjaran. Jl. Ir. Soekarno Km 21, Jatinangor, Sumedang 45363, West Java, Indonesia Manuscript received: 30 March 2017. Revision accepted: 14 June 2017. Abstract. Kuntana YP, Yurmiati H, Wulandari AP, Syafitri F, Partasasmita R. 2017. The appearance of rabbit skin tissue (Oryctolagus cuniculus) after supplementation of Aloe vera and Spirulina fusiformis. Nusantara Bioscience 9: 268-274. The research is about the appearance of rabbit skin tissue (Oryctolagus cuniculus L.) after supplementation of Aloe vera L. and Spirulina fusiformis Vor. has been done. This study was carried out to find the most effective formulation ofsupplement of A. vera and S. fusiformis as a natural supplement that can generate the best appearance of rabbit skin tissue. The method in this research was a single Complete Random Design (CRD) on 28 male New Zealand White strains rabbits with the age of 16 weeks. The treatments were divided into seven groups with four replications, namely, P 0 (negative control ), P 1 (positive control, vitamin C19 mg/kg BW), P 2 (A. vera 74 mg/kg BW), P 3 (S. fusiformis 296 mg/kg BW), P 4 (A. vera: S. fusiformis, 74: 148 mg/kg BW), P 5 (A. vera: S. fusiformis, 74: 296 mg/kg BW), and P 6 (A. vera: S. fusiformis, 74: 593 mg/kg BW). The parameters observed were production aspect (hair texture, slaughter weight, skin width, and skin weight percentage) and histological aspect (thickness of skin tissue and the amount of hair follicle). All of the data was analyzed using ANOVA test (P ˃ 95%) and Duncan test (P ˃ 95%). The result showed that the giving of supplement with the basis of the formulation of A. vera 74 mg/kg bw and S. fusiformis 296 mg/kg bw was effective in generating the best appearance of rabbit skin tissue. Keywords: Aloe vera, Appearance of skin tissue, Rabbit, Spirulina fusiformis INTRODUCTION The skin raw materials commonly used for various types of clothing and food products are derived from cattle, buffalo, sheep, and goats. The skin produced from cattle has good quality but, has relatively low reproductive rate and high production costs lead to limited provision. One attempt to solve this problem is to develop a type of livestock that has high reproduction and low production costs, such as rabbit. This time, the rabbit skin is just a waste of the ranch. The rabbit skin is one alternative that has great potential to be processed into useful products such as jackets, bags, carpets and toys, in addition, the hair fibers can be developed into wool. The skin quality is closely related to feeding and enclosure management. The skin width covering the body surface will increase with weight gain. The increase of body volume will be followed by increasing of chest size circumference and body length so that it can affect the width and length of the skin (pelt). Pelt is a fresh skin tissue of furry animals that has been skinned. Pelt thickness is related to fat content, the layers of epidermis, dermis and connective tissue. Food is needed by rabbit for the production of wool and pelts like Angora and Rex which need 120 g/day with 15% of crude protein. Nowadays, rabbit ranch uses expensive pellet with high protein (16%), thus it gives sufficient natural supplement for rabbits. Spirulina was chosen as one of the alternative natural supplement because it has several advantages such as high protein which is up to 60-70% of the entire dry weight, containing essential fatty acids, polysaccharides, carotenoids, vitamins, and minerals, especially vitamin B12 (Bourges et al. 1971; Anusuya et al. 1981; Kabinawa 2014; El-Tantawy 2015). The contents of minerals and vitamins in Spirulina are potassium (15,400 mg/kg), calcium (1,315 mg/kg), zinc (39 mg/kg), magnesium (1915 mg/kg), manganese (25 mg/kg), iron (580 mg/kg), selenium (0.40 ppm), phosphorus (8942 mg/kg), as well as vitamins A, B1 (thiamine), B2 (riboflavin), B3 (nicotinamide), B6 (pyridoxine), B9 (sour folate), B12, vitamin C, vitamin D and vitamin E. The complete and balanced of Spirulina nutritional content has been used optimally in some countries to overcome malnutrition and immune system. The supplementation of Spirulina dose of 800 mg/kg body weight (BW) of mice is proven to improve liver function and to repair kidney and testicular damage due to mercury exposition. The dosage is converted to rabbits consumption and it becomes 296 mg/kg BW (Hermosillo et al. 2011; Henrikson 2009; Susanna et al. 2007. The plant of A.vera is one of the herbs. This plant contains two types of liquids, namely a clear liquid which