_____________________________________________________________________________________________________ *Corresponding author: Email: m.wahyudin.fikri@gmail.com; Asian Journal of Fisheries and Aquatic Research 15(4): 1-10, 2021; Article no.AJFAR.73271 ISSN: 2582-3760 The Used of Natural Anesthetic Materials Nutmeg Seed Oil in the Transportation of Sumatran Fish Fingerlings (Puntius tetrazona) Walim Lili 1 , Muhamad Fikri Wahyudin 1* , Asep Agus Handaka Suryana 1 and Kiki Haetami 1 1 Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Indonesia. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJFAR/2021/v15i430333 Editor(s): (1) Rakpong Petkam, Khon Kaen University, Thailand. Reviewers: (1) Yitayal Admassu Workie, Addis Ababa Science and Technology University, Ethiopia. (2) Hery Muhamad Ansory, Universitas Setia Budi, Indonesia. Complete Peer review History: https://www.sdiarticle4.com/review-history/73271 Received 02 August 2021 Accepted 04 October 2021 Published 06 November 2021 ABSTRACT The low survival rate in Sumatran fish fingerlings transportation is a problem related to metabolic disturbances that cause death. The addition of nutmeg seed oil in the transportation medium is expected to reduce the rate of respiration and metabolism, so that fish mortality can be minimized. The purpose of this study was to determine the effective concentration of nutmeg seed oil to maintain the highest survival rate of Sumatran fish fry transported during treatment and to analyze its effect on induction time and recovery time. This research was carried out from January to March 2021 in the Ciparanje wet laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research was conducted experimentally using a factorial randomized group design (FRGD) consisting of two factors, namely the concentration of four levels (0.03, 0.06, 0.09 ml/L and control) and duration of three levels (3, 5, and 7 hours) which was repeated three times. This research uses a closed transportation system. Parameters observed were induction time and conscious recovery time of test fish, post-transportation and post-maintenance survival for 7 days and water quality consisting of temperature, DO, pH, and ammonia. The results showed that the effective nutmeg seed oil for the anesthesia of Sumatran fish fry transported was 0.03 ml/L with a transportation duration of 3 hours because it had an induction time of 08:07, recovery time of 02:41 Original Research Article