Proceeding of 1 st Unnes International Conference on Research Innovation & Commercialization for the Better Life 2015 ISSN: 2460-5832 November 27-28 th , 2015, Semarang, Indonesia 241 conf.unnes.ac.id/index.php/uicric Exploration bacteria trash of Rawasari and Bantar Gebang Indonesia as a superior candidat of compost bacterial agent Rini Puspitaningrum 1 , Mohamad Irfan 1 , Mohamad Isnin Noer 1 , Gilang Ainan 1 , Ria Amelia 1 , Gladies Neolaka 1 , Shuhei Yabe 2 , Yasuteru Sakai 2 , Masaru Hazaka 2 , Dalia Sukmawati 1 , Muzajjanah 1 , Yokota Akira 2 1. Department of Biologi Faculty of Math and Natural Sciences, Universitas Negeri Jakarta (UNJ). Jl. Pemuda 10 Rawamangaun Jakarta13220 - Indonesia 2. Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza- Ashitate, Murata- cho, Shibata-gun, Miyagi 989-1311, Japan rini_puspitaningrum@yahoo.com Abstract- To obtain a superior compost bacterial agent carried out by exploration of bacteria that already exist in its place. This research is aimed at exploration bacteria trash from Rawasari and Bantar Gebang Indonesia. This descriptive research was exploration of bacteria in molecular level. The identification technique used consisted of observation on morphology and mineral analysis content of soil samples, molecular identification of 16sRNA genotype analysis using PCR and sequencing as well as kinship test bacteria using a program of Mega tree. The isolates multiplied using cloning techniques and analyzed its growth. Keywords-superior compost bacterial agent, Rawasari, Bantar Gebang, 16sRNA genotype identification. INTRODUCTION Application of waste by fermentation of microorganisms is one method of controlling garbage in the environment. Efforts waste processing using microorganisms will degrade organic waste into high quality compost. This study provides an important breakthrough in addressing the waste problem with the approach of exploration results and determination of seed compost bacteria in the fermentation process. microorganisms succession process in each stage and has a diversity of different microorganisms (Peters et al. 2000). Succession is categorized into four stages based diversitasnya, namely: mesophilic, thermophilic, mesophilic second, and maturation (Ryckeboer et al. 2003). Fermentation is the result of acid hydrolysis of fats and also as a result of the activity of bacterial growth. One of the organic acids resulting from the process is lactic acid. Lactic acid is formed as a result of anaerobic glycolysis metabolism through enzymatic conversion of pyruvate by the help of the enzyme lactate dehydrogenase - LDH. Lactic acid may increase the acceleration revamp organic materials such as lignin and cellulose and memfermentasikannya without causing toxic compounds Microorganisms can be bacteria that produce lactic acid is known for Lactic Acid Bacteria (LAB). Lactic acid bacteria can ferment carbohydrates to produce lactic acid. Functional lactic acid bacteria decompose organic matter by fermentation forming lactic acid and glucose. The lactic acid will suppress the growth of harmful microorganisms and improve the degradation of organic materials quickly (Javed et al. 2010) (Anif et al., 2007: 124). Bacillus bacteria under anaerobic conditions LDH enzyme plays an important role in forming ATP during glycolysis (Javed et al. 2010). Lactic bacteria isolated from compost is Bacillus sp. Bacillus sp. These bacteria are known most commonly found in the temperature range 20 ° C to 60 ° C, while above this range only strains of Bacillus stearothermophilus are able to live (Strom 1985). Bacillus sp. have a metabolic enzyme that plays a role in degrading cellulose, lignin, and bioplastics (Ryckeboer et al. 2003). Therefore, the role of Bacillus sp. very important in the beginning of the composting process. The genus Bacillus originally proposed by Cohn in 1872 were classified as bacteria which produce endospores (endospore-forming bacteria). This genus has a number of very fantastic kind reaches 146 species, including the two most popular types that B. anthracis and B. subtilis {Citation} (Fritze, 2004). Since Cohn, taxonomy Bacillus undergone some changes, until now known only valid genus Bacillussecara teradapat 88 species (Fritze, 2004) and about 18 species ditemukanpada compost (Ryckeboer et al., 2003). The purpose of this research is to isolate new strains of mesophilic bacteria that get into the genus Bacillus sp. of compost in Bantargebang (Indonesia), namely Bacillus sp. strain-BG, define the taxonomic and phylogenetic position of organisms through a complete analysis area 16 rRNA. This research will be conducted identity and combinations LDH isoform composition of Bacillus sp. METHODS Samples taken from the soil bacterium derived from the land end of the garbage collection population in Jakarta, namely Bantargebang - Bekasi Indonesia and land waste collection in the area of Rawasari- East Jakarta. We have done in Hazaka Plant Research Center were sampling, making media, sterilization using autoclave, direct inoculation, and sample incubation. Furthermore, bacterial samples are stored in the Laboratory of Microbiology, Faculty of mathematics and natural science Universitas Negeri Jakarta. Bacteria Culturing Stage