IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 11, Issue 3 (Nov.- Dec. 2013), PP 63-66 www.iosrjournals.org www.iosrjournals.org 63 | Page Study of Seroprevalence of HIV, Hepatits B and C And Syphilis Among Blood Donors In A Tertiary Care Hospital, Kolkata Dr. Sukla Naskar 1 , Dr. Sumit Nandy 2 , Prof. Keya Basu 3 , Dr. Rivu Basu 4 1 Assistant Professor, Dept of Pathology, Calcutta National Medical College, Kolkata, West Bengal, India 2 Demonstrator, Dept of Pathology, Calcutta National Medical College 3 Professor and Head, Dept of Pathology, Calcutta National Medical College 4 Assistant Professor, Dept of Community Medicine, R G Kar Medical College, Kolkata Abstract: Transfusion transmitted infections (TTIs) are a real concern worldwide. Blood transfusion is an important mode of transmission of infections to recipients. The aim of our study was to assess the prevalence of transfusion transmitted infection among blood donors, a 10years retrospective study, from April 2002 to March2012 was conducted at the blood bank of Calcutta National Medical College and Hospital, Kolkata, West Bengal. Donors were screened routinely for seroprevalence of HIV, HBV, HCV and syphilis and malaria. Our study was focused on mainly HIV, Hepatitis and syphilis. A total of 128119 donors were tested, out of which 523 (0.40%) were replacement donors and 127596 (99.59%) were voluntary donors. The seroprevalence of HIV was 0.28% in the donors. The seroprevalence of HBV, HCV and syphilis was 1.75%, 0.37% and 0.44% respectively in total donors. Seroprevalence of HBV, HCV, and HIV in voluntary blood donors is high throughout this study. Key words: TTI, Blood donors, Hepatitis, HIV, Seroprevalence, Prevention I. Introduction: Blood transfusion forms an integral part of modern mode of therapy. Transmission of infectious diseases through donated blood is of concern to blood safety. Blood transfusion carries the risk of transfusion- transmissible infections, including HIV, hepatitis, syphilis, malaria and infrequently toxoplasmosis, and some viral infections like CMV, EBV and herpes. With every unit of blood, there is 1% chance of transfusion- associated problems including transfusion-transmitted diseases. [1] Among the TTIs, hepatitis B (HBV), hepatitis C (HCV), and HIV are the most dreadful. 2 billion people have been infected with HBV and 360 million have chronic infection worldwide and it is the 10 th leading cause of death worldwide causing 500000 to 1.2 million deaths per year due to chronic hepatitis, cirrhosis, and hepatocellular carcinoma. [2],[3] In Asia and most of Africa, chronic HBV infection is common and in Western countries, the disease is relatively rare. [3] Nearly 3.9 million people are estimated to be infected with HCV, the most common chronic blood-borne infection, leading to 8000 to 10000 deaths annually in USA. [4] The Indian subcontinent is classified as an intermediate HBV endemic (HBsAg carriage 2-7%) zone and has the second largest global pool of chronic HBV infections. [3] The improved screening and testing of blood donors has significantly reduced transfusion-transmitted diseases in most developed countries. This has not been so in developing nations. Poor health education and lack of awareness result in the reservoir of infections in the population. The aim of our study was to know the seroprevalence of transfusion-transmitted infections in donors screened in blood bank in CNMCH, Kolkata. II. Materials And Methods: This cross-sectional observational study was done among voluntary and replacement blood donors who attended blood bank of CNMCH or blood donation camps during the period of April 2002 to March 2012. In this we included followings : (1) subjects of either sex, (2) age group (in years) - 18 to 60, and (3) subjects otherwise healthy for blood donation. The screening for HIV was done by ELISA using kits (Monozyme; J. Mitra, India). Hb S Ag was detected by ELISA (Transasia, India and J. Mitra, India). Anti-HCV test was done by ELISA (Monozyme, J Mitra, India and Transasia, India). Test for syphilis was done by RPR (Mediclone, India and Tulip, India). Statistical analysis done by Chi-square test where p value less than 0.001.