Open Access Maced J Med Sci. 2021 Apr 22; 9(A):183-188. 183 Scientifc Foundation SPIROSKI, Skopje, Republic of Macedonia Open Access Macedonian Journal of Medical Sciences. 2021 Apr 22 9(A):183-188. https://doi.org/10.3889/oamjms.2021.5982 eISSN: 1857-9655 Category: A - Basic Sciences Section: Pharmacology Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena Odorata L.) Using DPPH and ABTS Methods Karlina Amir Tahir 1,5 *, Upik A. Miskad 2 , Khairuddin Djawad 3 , Sartini Sartini 4 , Natsir Djide 4 , Khaerani Khaerani 1 , Maulita Indrisari 5 1 Department of Pharmacy, Faculty of Medicine and Health Sciences, UIN Alauddin Makassar, Indonesia; 2 Department of Anatomical Pathology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; 3 Department of Dermatology and Venereology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; 4 Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia; 5 Postgraduate Doctoral Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia Abstract BACKGROUND: Antioxidants are substances that can inhibit the oxidation process, so that they have a protective efect on cells from the dangers of free radicals. One of the plants that has activity as natural antioxidants is Botto- Botto leaves (Chromolaena odorata L.). AIM: This study aims to determine the antioxidant activity of the Botto-Botto leaf fraction using the DPPH and ABTS methods. METHODS: The leaves of Botto-Botto (Chromolaena odorata L.) obtained from Takalar District, South Sulawesi are sorted, dried and pollinated. The powder was immersed in 70% ethanol solvent and sonicated. Furthermore, the extraction was carried out by maceration method with 70% ethanol solvent and fractionated using water, n-hexane, and n-butanol as solvents and dried. The fractionation results were continued for antioxidant testing using the DPPH and ABTS methods. RESULTS: The n-butanol fraction of Botto-Botto leaves had the highest antioxidant activity, namely the strong active category. In the DPPH and ABTS methods, the n-butanol fraction has IC50 values of 33.535 µg / mL and 60.885 µg / mL, respectively. CONCLUSION: The DPPH and ABTS methods, the n-butanol fraction of botto-botto leaves have strong antioxidant activity compared to other fractions, namely the water fraction and the n-hexane fraction. Edited by: Sinisa Stojanoski Citation: Tahir KA, Miskad UA, Djawad K, Sartini S, Djide N, Khaerani K, Indrisari M. Analysis of Phytochemical Substances and Antioxidant Activity of Botto-Botto Leaves (Chromolaena odorata L.). Open Access Maced J Med Sci. 2021 Apr 22; 9(A):183-188. https://doi.org/10.3889/ oamjms.2021.5982 Keywords: Antioxidant; Botto-Botto (Chromolaena odorata L.); 1-diphenyl-2-picryl-hydrazyl; 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid diammonium salt *Correspondence: Karlina Amir Tahir, Department of Pharmacy, Faculty of Medicine and Health Sciences, Alauddin State Islamic University, Makassar, Indonesia. E-mail: karlina.amir@uin-alauddin.ac.id Received: 07-Mar-2021 Revised: 01-Apr-2021 Accepted: 14-Apr-2021 Copyright: © 2021 Karlina Amir Tahir, Upik A. Miskad, Khairuddin Djawad, Sartini Sartini, Natsir Djide, Khaerani Khaerani, Maulita Indrisari Funding: This study was supported by the 5000 Doctoral Scholarship Program in 2018 Competing Interest: The authors have declared that no competing interest exists Open Access: This is an open-access article distributed under the terms of the Creative Commons Attribution- NonCommercial 4.0 International License (CC BY-NC 4.0) Introduction The medicinal properties of plants are caused by the active chemical elements present in various plant parts. This chemical element has a physiological efect on the human body. The most important bioactive compounds from plants are alkaloids, favonoids, tannins, and phenolics. Phytochemical research based on ethnopharmacological information is generally considered a practical approach to discovering new antioxidants from higher plants [1], [2], [3]. Antioxidants are broadly defned as substances that inhibit the oxidation process from protecting cells from the dangers of free radicals that arise from body metabolism or other external factors. Chemically, natural antioxidants found in plants and foodstufs are mainly derived from phenol-derived compounds such as favonoids (quercetin) [4], [5], [6]. Chromolaena odorata (Family: Asteraceae) is synonymous with Eupatorium odoratum, a traditional medicinal plant widely used for its wound healing properties. Some parts of this plant have been used to treat wounds, burns, and skin infections [7]. Furthermore, this plant has been shown to have an anticancer, antidiabetic, antihepatotoxic, anti-infammatory, antimicrobial, and antioxidant profle [8]. The phytochemical components are alkaloids, favonoids, favanones, essential oils, phenolics, saponins, tannins, and terpenoids. Other important constituents of this plant are Eupolin, chromomoric acid, quercetin, and quercetin. The efciency of wound healing comes from the drug or plant’s antioxidant properties, which increase the preservation of fbroblasts and the proliferation of keratinocytes in the wound [9], [10], [11]. Oxidation is an essential process in living things. Free radicals are produced from metabolic pathways or environmental sources that interact continuously with biological systems. Reactive oxygen species (ROS) are the most signifcant primary catalysts that initiate the oxidation process in vivo and in vitro and generate oxidative stress [12]. Antioxidants have the ability to destroy free radicals and maintain body cells, including immune cells, from oxidation stress. The potential antioxidant efects of these plants are