Int.J.Curr.Microbiol.App.Sci (2017) 6(12): 1972-1977 1972 Original Research Article https://doi.org/10.20546/ijcmas.2017.612.226 A Study on Biofilm Phenotype and Antimicrobial Resistance Pattern of Clinical Isolates from Catheter Associated Urinary Tract Infection Neha Haswani*, Tasmiya Katheeja and Sathya Anandam Department of Microbiology, Yenepoya Medical College, university road, Deralakatte, Mangalore-575018, Karnataka, India *Corresponding author ABSTRACT Introduction Biofilm is a sessile microbial community which is characterized by the cells having capacity to irreversibly attach to the substratum (either animate or inanimate) or to each other and being embedded in a matrix of self-produced extracellular polymeric substances (Donlan et al., 2002). Biofilms enhance the virulence of an organism, thereby rendering a less virulent organism or an avirulent microbe with huge power of being virulent 2 . They are currently estimated to be responsible for over 65% of nosocomial infections and 80% of all microbial infections (Romling et al., 2012). Catheter associated urinary tract infection (CAUTI) is a common health care associated infection throughout the world, occurring due to one common reason of magnanimous usage of indwelling urinary catheters teamed with inappropriate antibiotic use (Majumder et al., 2014). The mechanism of such infections is mostly due to the inert nature of the catheter material which is susceptible to microbial colonization and formation of biofilm, thus enhancing the pathogenic effects (Trautner and Darouiche, 2004). Medically, biofilms decrease the antimicrobial susceptibility and render treatment difficult. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 12 (2017) pp. 1972-1977 Journal homepage: http://www.ijcmas.com Biofilms associated with indwelling medical devices are a major public health concern now-a-days as they are difficult to treat with the routine antibiotics. So the present study was taken up to study the biofilm producing phenotype and antimicrobial resistance pattern of pathogens in patients with catheter associate urinary tract infection (CAUTI). Method: this prospective study with 50 urine samples from catheterized patients (indwelling urinary catheter of more than 48 hrs) was carried out over a period of three months. Isolation and identification of the organism was followed by detection of biofilm phenotype by the tissue culture plate method. The results were analysed using SSPS 22.0 software. The most common organism isolated was E. coli (54%), followed by Klebsiella pneumoniae (16%). Among the total isolates, 31 (62%) were positive for the biofilm phenotype. Biofilm producing strains showed relatively higher antibiotic resistance than non-producers. 71% of the biofilm producers were multidrug resistant. Our study showed a significant association between biofilm production and multidrug resistant. Keywords Catheter associated urinary tract infections, Biofilm, Tissue culture plate method. Accepted: 15 October 2017 Available Online: 10 December 2017 Article Info