Research Article Open Access
Volume 5 • Issue 6 • 1000279
Med Aromat Plants (Los Angel), an open access journal
ISSN: 2167-0412
Open Access Commentary
Rajput et al., Med Aromat Plants (Los Angel) 2016, 5:6
DOI: 10.4172/2167-0412.1000279
*Corresponding author: Ratnamala S Bendre, School of Chemical
Sciences, North Maharashtra University, Jalgaon, Maharashtra, India, Tel:
+919422211435/+912572257435; E-mail: bendrers@gmail.com
Received February 07, 2016; Accepted December 16, 2016; Published
December 20, 2016
Citation: Rajput JD, Bagul SD, Tadavi S, Bendre RS (2016) Comparative Anti-
Proliferative Studies of Natural Phenolic Monoterpenoids on Human Malignant
Tumour Cells. Med Aromat Plants (Los Angel) 5: 279. doi: 10.4172/2167-
0412.1000279
Copyright: © 2016 Rajput JD, et al. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited.
Keywords: SRB; Carvacrol thymol; Eugenol; MCF-7: SK-MEL-2;
HCT-15; MIAPaCa-2
Introduction
Cancer is a serious chronic disease that arises due to changes in
many physiological processes in body [1,2]. In general, symbols of
cancer were found to be sustaining proliferative signalling, avoiding
growth suppressors, control cell death, enabling replicative immortality,
inducing angiogenesis, and activating incursion and metastasis,
along with two emerging symbols including reprogramming energy
metabolism and absconding immune damage [3-5]. Te search for
novel small molecules as drugs is still a priority goal for cancer therapy,
due to the rapid development of resistance to chemotherapeutic
drugs [6]. In addition, the high toxicity usually associated with some
bulky anticancer drugs and their undesirable side-efects increase
the demand for novel anti-tumour drugs active towards untreatable
tumours [7,8] Natural products have long been a substantial source
of treatment for melanoma, which is projected to become the main
causes of death in this century [9,10]. Te research of last 40 years
demonstrated that more than one thousand plants possess signifcant
anticancer properties [11]. while many molecules obtained from these
plants have shown wonderful anticancer properties [12,13]. Some of
them showed efective delivery in biological system to reduce the action
of cancer disease, without toxic and other side efects against healthy
cells and tissues [14].
Carvacrol (1), thymol (2) and eugenol (3) are part of a naturally
occurring class of compounds known as phenols [15]. Tey are
well-known naturally occurring phenolic monoterpenoids found
primarily in oils of oregano, thyme, and marjoram, and are recognized
as traditional therapeutic agents [16,17]. All the major groups of
angiosperms biosynthesize these biocides [18]. Currently, these
biocides are used in food favouring ingredients and preservatives,
as well as a fragrance ingredients in cosmetic formulations [19]. In
recent years, signifcant research has been undertaken as an efort to
establish the biological actions of these phenolic monoterpenoids for
their potential use in pharmaceutical and agricultural applications [20].
Some results from in-vitro and in-vivo studies showed that carvacrol,
Comparative Anti-Proliferative Studies of Natural Phenolic
Monoterpenoids on Human Malignant Tumour Cells
Jamatsing D Rajput, Suresh D Bagul, Samina Tadavi and Ratnamala S Bendre*
School of Chemical Sciences, North Maharashtra University, Jalgaon, Maharashtra, India
Abstract
In present study we report prominent effect of carvacrol (5-isopropyl-2-methyl phenol), thymol (2-isopropyl-5-
methyl phenol) and eugenol (4 allyl-2-methoxyphenol) on Human melanoma cells. These three compounds were
screened for Anti-proliferative test by using Sulforhodamine B (SRB) assay. For this investigation we used human
breast melanoma cell line MCF-7, human skin melanoma cell line SK-MEL-2, human colon melanoma cell line
HCT-15 and human pancreatic melanoma cell line MIAPaCa-2. Carvacrol was identifed as most potent molecule
with low GI
50
value as 0.1 μL for selected human cancer cell lines; which is comparatively equal to standard
drug 14 Adriamycin (ADR). Thymol and Eugenol also exhibited remarkable GI
50
for human cancer cell 15 lines.
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ISSN: 2167-0412
Medicinal & Aromatic Plants