Aquacultura Indonesiana (2020) 21(2): 82-86 ISSN : 2477-6939 AQUACULTURA INDONESIANA An International Journal of Indonesian Aquaculture Society (www.aquasiana.org) © Copyright by Indonesian Aquaculture Society 2020 82 Inhibition Activity In Vitro Test of Temu Kunci Extract (Boesenbergia Pandurata) againts Streptococcus sp. and Aerococcus sp. Wey Ninta Carina 1 , Esti Handayani Hardi 2 , Henny Pagoray 3 1 Study Program of Aquaculture, Faculty of Fisheries, University of Mulawarman Samarinda 2,3 Departement of Aquaculture, Faculty of Fisheries, University of Mulawarman Samarinda, Indonesia Corresponding email : weyninta1606015002@gmail.com Received : 13 July 2020 , Accepted : 30 December 2020 Abstract Wey Ninta Carina, Esti Handayani Hardi, and Henny Pagoray. 2020. Inhibition Activity In Vitro Test of Temu Kunci Extract (Boesenbergia Pandurata) againts Streptococcus sp. and Aerococcus sp. . Aquacultura Indonesiana, 21 (2): 82-86. This study examines the effective concentration of temu kunci extract (Bosenbergia pandurata) as an antibacterial inhibition zone against 3 Aerococcus sp. isolates. and 2 Streptococus sp. isolates. The method used is the ADD (Agar Diffusion Disc) method and bacterial culture together with the bacterial count using the TPC (Total Plate Count) method. Extract concentrations used were concentrations of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm, 900 ppm, and 1000 ppm. Positive controls used were amoxicillin concentration of 500 ppm and negative controls using sterile aquades. Bacteria were grown on BHIB media and then as much as 1 ml were spread on BHIA media on petri dishes, then 20 μl drops were dropped on an antibacterial disc measuring 6 mm, then incubated at 30ºC for 48 hours and carried out also for 24 hours and 48 hours. Furthermore, in the second experiment carried out by culture together the extract concentrations used were 600 and 800 ppm, the results showed that the concentration of 800 ppm was greater than the growth of bacterial destruction by the ADD method and the TPC method and the zone of inhibition of the extract against the test bacteria 10.61- 13.47 mm. Keywords: Temu Kunci (B.pandurata), Aerococcus sp., Streptococcus sp., Zone of Inhibition Introduction Enlarging aquaculture activities can be decreased by various problems. One of the problems in fish farming is the coming bacterial diseases. Based on the research results done Hardi et al in 2018, it was found 37 types of bacteria being capable of infecting cultured fish. fourteen of these bacteria are suspected to be pathogenic to both humans and aquaculture animals. Two bacteria are called Aerococcus sp. and Streptococcus sp. Aerococcus sp. is a bacterial pathogen which is one of its varieties, named Aerococcus viridans var. Homaries. It causes disease agents of gaffchemia and systemic homarid lobsters (Homarus americanus and H. gammarus). They are found in coastal waters of North America, Korea, Europe (Wood, 1999). The effect of gaffkaemia infection on lobsters is that the lobsters appear lethargic, drooping tails, and pale pinks on the sides of the stomach, lobsters swim sideways, and can lose balance when swimming (Lewbart, 2006). Bacteria Streptococcus sp. is bacteria causing infectious diseases in several types of freshwater fish and sea fish. The disease known as Streptococcosis is also a main problem in tilapia farming. The difference in appearing symptoms can be related to the target organ of S. agalactiae (eyes, brain and kidney). The presence of bacteria in the eye organ can produce changes in the eye (purulence, opacity, exoptalmia and shrinkage of the eye). The presence of bacteria in the brain organs can cause fish swim abnormally (gasping, swimming sideways even whirling) while the presence of bacteria in fish kidneys can cause discoloration (Hardi et al., 2011) Antibiotics have an important role in the world of health, antibiotics are expected to kill the bacteria causing infection (Jeffrey, 2006). The use of antibiotics must consider several important factors, including the selection of types and dosages of antibiotics, because the wrong use of can lead to new problem called resistance (Erviani, 2013). plant antibiotics can be used as an alternative for the resistence (Jaksa, 2009) Boesenbergia pandurata extract contains essential oils. Essential oil or etheric oil (essential oil, volatile) is deriving from the http://dx.doi.org/10.21534/ai.v21i2.202