Int.J.Curr.Microbiol.App.Sci (2017) 6(9): 2319-2328 2319 Original Research Article https://doi.org/10.20546/ijcmas.2017.609.284 Native Trichoderma for the Management of Wire Stem of mustard (Brassica spp) Caused by Rhizoctonia solani R.K. Nirupama Devi, Bireswar Sinha* and Ph. Sobita Devi Department of Plant Pathology, College of agriculture, CAU, Imphal, Manipur, India *Corresponding author ABSTRACT Introduction Mustard (Brassica sp.) is the important Rabi oilseed crops of India. They occupy a prominent place being next to groundnut, both in area and production. It is considered to be a crop of tropical as well as temperate zone and require relatively cool temperature for satisfactory growth and yield. It belongs to genus Brassica of the family Cruciferous with large number of species and sub species cultivated in India. Brassica juncea L. was originally introduced from China into North- Eastern India, from where it has extended into Afghanistan via Punjab, Eastern Afghanistan, together with the adjoining North-Western India is one of the independent centres of origin of brown sarson (Brassica campestris var. brown sarson). India is one of the largest rapeseed mustard growing countries in the world occupying the first position in area and second position in production after China. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 9 (2017) pp. 2319-2328 Journal homepage: http://www.ijcmas.com In vitro study on the effect of four Trichoderma isolates on the mycelial growth of Rhizoctonia solani showed maximum inhibition of 74.54 per cent and least inhibition of 52.50 per cent. Effects of volatile compound produced by Trichoderma spp. against R. solani were ranged from 15.50 to 26.70 per cent. Non-volatile compound produced by Trichoderma spp showed inhibition of 7.45 to 31.11 per cent at 7.5 % v/v concentrations and 14.50 to 33.55 per cent at 15 % v/v. Experiment on competitive parasitic ability of four Trichoderma isolates in the colonization of sclerotia under three different soil conditions viz., unsterilized, sundried and sterilized soil showed highest colonization of isolates CAUNCIPM-116, CAUNCIPM-97A, CAUNCIPM-11 and CAUNCIPM-50 (100 per cent) sterilized soil. Bio priming of mustard seeds by Trichoderma isolates showed highest shoot length of 4.90 cm by Trichoderma isolate CAUNCIPM-97A and highest root length of 1.47cm in isolate CAUNCIPM-11. Highest vigour index was observed in isolate CAUNCIPM-97A treated seed. Fungal microbial population from the rhizosphere of mustard was taken at 15 days and 30 days after sowing and highest number of fungal population was observed in seed + soil treated with isolate CAUNCIPM-97A and CAUNCIPM-116 respectively. In vivo efficacy of four isolates viz., CAUNCIPM-116, CAUNCIPM-97A, CAUNCIPM-11 and CAUNCIPM-50 of Trichoderma against R. solani Kuhn. showed significant reduction in disease incidences under pot condition and beside chemical (Blitox) treated pot, combination of seed and soil treatment with Trichoderma isolates showed highest plant growth characters pot such as germination percentage, plant height, plant canopy, fresh weight and dry weight than seed and soil alone. Keywords Mustard, Trichoderma, Bio- priming. Accepted: 23 August 2017 Available Online: 10 September 2017 Article Info