C Characterization o Candida Arti S. Shanware Rajiv Gandhi Biotechnology C Laxminarayan Institute of Technolo Rashtrasant Tukdoji Maharaj Nagpu Amaravati Road, Nagpur - 440033, Ma E-mail: artishanware@gmail Tel.: 0712-2560620. 2536223; Fax: 0712- Abstract – The rhizospheric speci Pseudomonas are the eminent biological co are not only known for alternative to controlling plant diseases but also prom Amongst the PGPRs, fluorescent Pseudom as the largest and potentially the most p PGPR, because of their potential to act a biofertilizing agents. In the present Pseudomonas (FP) isolates FP-1, FP-2 and from the rhizospheric soil of Cotton Shivasawanga located in Nagpur distr India. The isolates were subjected to stand microbiological, biochemical profilin characterized for molecular analysis. O rRNA analysis, nucleotide homology analysis the strains FP-1, FP-2 and FP-3 Pseudomonas fluorescens strains. The developing liquid biofertilizer by using strains of fluorescent Pseudomonas. By liquid biofertilizer, the better yield of cro region can be achieved. Keywords – Biological Control, Fluores PGPR, Rhizobacteria, Antagonism. I. INTRODUCTION Biological method for control of plan a highly effective, economical and envir alternative to the use of synthetic ferti intensive use of chemical fertilizers for p against phytopathogens which are develo plant pathogens as well as demolishin hence the use of biological methods approach in modern agriculture pract naturally occurring soil organisms inc Pseudomonas promote the growth of variety of mechanisms. Fluorescent Pseu to plant growth promoting rhizobacter actively colonize plant roots and promo direct or indirect mechanism [5]. Dire plant growth includes production of ph solubilization of phosphate and other m their indirect mechanism includes produ [8] and siderophore [9] which are phytopathogens. Fluorescent Pseud biocontrol by production of antibiot diacetylphloroglucinol (PHL), pyo pyrrolnitrin (PRN), phenazine-1-carbox 2- hydroxy phenazines and phenazi Copyright © 2014 IJAIR, All right reserved 1168 International Journal of Agriculture I Volume 2, Issue 6, of Fluorescent Pseudomon ates for Liquid Bioinocula Centre, ogy, Campus, ur University, aharashtra, India l.com 2545781, 2532841 Ashwini S. D Rajiv Gandhi Biotec Laxminarayan Institute of Rashtrasant Tukdoji Mahar Amaravati Road, Nagpur - 44 ies of fluorescent ontrol agents. These agrochemicals for mote plant growth. monas have emerged promising group of as a biocontrol and study fluorescent d FP-3 were isolated n fields of village rict, Maharashtra, dard morphological, ng and further On the basis of 16S and phylogenetic 3 were identified as e study aims at g some competent y formulating such ops in the Vidarbha scent Pseudomonas, nt pathogens offers ronmental friendly ilizers [1]-[2]. The protection of crops oping resistance in ng the soil health, s is a significant tices [3]-[4]. The cluding fluorescent f host plant by a udomonas belongs ria (PGPR), which ote plant growth by ect mechanism of hytoharmones [6], minerals [7], while uction of antibiotics e suppressive to domonas achieve tics such as 2,4- oluteorin (PLT), xyclic acid (PCA), ine-1-carboxamide (PCN) [10]. These antibiotics p management as they inhibit t concentration. In addition, to pr they also induce a systemic resi The use of biological control diseases is one of the mos sustainable agriculture de Pseudomonas spp. offer an exc range of phytopathogenic bact that rhizospheric bacteria of a efficient diseases controller isolated from other plant sp screening of locally adapted bi [5]. The present study characterization and evaluatio fluorescent Pseudomonas spp. commercial world. II. MATERIALS A 2.1. Soil sampling and i bacteria Root adhering soil samples w plant rhizospheric region grow which is located near Nagpu prepared by shaking 1g of so undamaged root pieces with tig of sterile distilled water and kep to release the rhizoplane bacte were serially diluted from 10 -1 suspension was spread on to K agar plate and incubated at 28º of fluorescent Pseudomonas wa (356 nm) by using Transilluminator. On these prel three isolates namely FP1, FP fluorescent Pseudomonas which light ( fig. 1). Fig.1. Fluorescent Pseudomon Manuscript Processing Details (dd/mm/yyyy Received : 06/06/2014 | Accepted on : 24/06 Innovations and Research , ISSN (Online) 2319-1473 nas: Potential ant Darokar chnology Centre, Technology, Campus, raj Nagpur University, 40033, Maharashtra, India play a critical role in disease the phytopathogens at low roduce antimicrobial agents istance in plants [10]. agents for controlling plant st promising method for evelopment. Fluorescent cellent biocontrol against a teria and fungi. It is noted particular crop may be the than bacteria originally pecies [11]-[5]. Therefore, iocontrol strains is essential is based on isolation, on of potential strains of . for their intensive use in AND METHODS isolation of fluorescent were collected from Cotton wn in field of Shivasawanga ur. A soil suspension was oil sample having 2-3 cm ghtly adhered soil in 100 ml pt for 1hr on a rotary shaker eria. The processed samples to 10 -6 and 0.1 ml of the King’s medium B ( KMB ) ºC for 48h. The occurrence as examined under UV light Spectroline Ultravoilet liminary identification basis P2, FP3 were identified as h fluorescenced under UV- nas isolates under UV light y) : 6/2014 | Published : 29/06/2014