J Med Sci, Volume 49, No. 3, 2017 July: 114-120 114 Corresponding author: mega.febia.s@mail.ugm.ac.id J Med Sci, Volume 49, No. 3, 2017 July: 114-120 Effect of ethanol extract of Syzygium cumini (L.) pulp on blood pressure and malondyaldehyde level of chronic restraint stress rats Mega Febia Suryajayanti 1 , Anggi Laksmita Dewi 1 , Bira Arumndari Nurrahma 1 , Ayu Dewi Silvia Putri 2 , Zunamilla Khairia 1 , Arta Farmawati 3 1 School of Nutrition & Health, 2 School of Nursing, 3 Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia DOI: http://dx.doi.org/10.19106/JMedSci004903201703 ABSTRACT Chronic stress disturbs the equilibrium of oxidant-antioxidant redox in the human body which accelerates cellular aging lead to promotes earlier onset of age-related diseases. To against the chronic stress human body needs exogenous antioxidant. Syzygium cumini (L.) has high antioxidant activity due to it’s high anthocyanin content. Therefore, this plant is potential to develop as a natural antioxidant. The aim of the study was to investigate the effect of ethanol extract of S. cumini (L.) pulp on blood pressure and malondialdehyde (MDA) level of rats induced by restraint stress. Twenty male Wistar rats were used in this study which divided into 4 groups with 5 rats in each group i.e. normal control, negative control and 2 treatment groups which given ethanol extract of S. cumini (L.) pulp at doses of 100 and 200 mg/g body weight (BW), respectively. Restraint stress was carried out by placing the rats in restraint rat for 30 minutes daily for 7 days. Blood pressure was measured before and after the treatment while MDA level was measured after the treatment. One-Way ANOVA was used to analyze the data. The results showed that induction of the restraint stress signifcantly increased blood pressure of the rats (p<0.001). The administration of ethanol extract of S. cumini (L.) pulp signifcantly prevented the increase in blood pressure of chronic restraint stress rats (p<0.001). Furthermore, the MDA level of the treatment groups was signifcantly lower than that of negative control (p<0.05) indicating that ethanol extract of S. cumini (L.) pulp could prevent the increase in the MDA level. In conclusion, administration of ethanol extract of S. cumini (L.) pulp can prevent the increase in blood pressure and MDA level of chronic restraint stress rats. ABSTRAK Stres kronik mengganggu keseimbangan senyawa oksidan-antioksidan yang memicu penuaan selular dan menyebabkan timbulnya penyakit yang berkaitan dengan penuaan lebih awal. Untuk melawan stress kronis tubuh memerlukan antioksidan oksidan. Syzygium cumini (L.) mempunyai aktivitas antioksidan yag tinggi karena kandungan antsianinya. Oleh Karena itu, tanaman ini potensial untuk dikembangkan sebagai antioksidan alami. Tujuan penelitian ini adalah mengkaji efek ekstrak etanol bubur S. cumini (L) terhadap tekanan darah dan kadar malondialdehid (MDA) tikus yang diinduksi stres dengan dikekang. Duapuluh tikus jantan Wistar digunakan dalam penelitian ini dibagi menjadi 4 kelompok