_____________________________________________________________________________________________________ *Corresponding author: E-mail: madhurishelar70@yahoo.com; Journal of Pharmaceutical Research International 33(47A): 691-697, 2021; Article no.JPRI.76119 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Development and Validation of HPTLC Method for Simultaneous Estimation of Resveratrol and Piperine in Its Pharmaceutical Dosage Form Ankita Gaikwad 1 , Madhuri Shelar 1* , Ganesh Andhale 1 and Jyoti Kadam 1 1 Department of Pharmaceutical Quality Assurance Technology, Alard College of Pharmacy, Pune- 411057, Maharashtra, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i47A33062 Editor(s): (1) Dr. Giuseppe Murdaca, University of Genoa, Italy. Reviewers: (1) Vinod Dhingra, India. (2) Emmanuel Obeagu, Imo State University, Nigeria. (3) Biswajit Dash, NEPEDS College of Pharmaceutical Sciences, India. Complete Peer review History: https://www.sdiarticle4.com/review-history/76119 Received 20 August 2021 Accepted 25 October 2021 Published 29 October 2021 ABSTRACT Resveratrol is a plant compound that work as antioxidant and it also has anti-aging properties whereas Piperine is a alkaloid and it majorly used in spices. A HPTLC (High performance thin layer chromatography) study is conducted for estimation of Resveratrol and Piperine. The Mobile phase used was Chloroform: Ethyl Acetate (50:50 v/v). Rf Value of Resveratrol 0.59 and Piperine 0.79 was found. Stationary phase of Silica gel 60 F 254 was used. Densitometric analysis was performed at 325 nm. The method was found to be linear. Recovery (ranging from 97% to 102.24 %), limit of detection (40.26 ng/spot, 1.64 ng/spot respectively for resveratrol & piperine), limit of quantification (122.02 ng/spot, 4.98 ng/spot respectively for resveratrol & piperine) and precision (≤ 2.00%) were found to be satisfactory. Validation performed with linearity, accuracy, precision, specificity, robustness, limit of detection and limit of quantification. Every parameter found within the range. The developed method allows to confirm that an accurate and reliable potency measurement of a pharmaceutical preparation can be performed. Keywords: HPTLC; piperine; resveratrol; validation. Original Research Article