IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.Volume 10, Issue 8 Ver. I (August. 2017), PP 10-13 www.iosrjournals.org DOI: 10.9790/5736-1008011013 www.iosrjournals.org 10 |Page Application of AAS in Study of Metal Cations Available In Leaf Extract of Some Medicinal Plants *Dhananjay Dwivedi 1 , Pallavee Gupta 1 1 (Department of Chemistry, P.M.B. Gujarati Science College, Indore, India) Corresponding Author: *Dhananjay Dwivedi Abstract: The plants selected for study is known to have Antidiabetic and Hepatoprotective properties. This study includes extraction of leaf content to know the level of metal cation (Chromium, Manganese, Iron, Nickel, Copper, Zinc and Cadmium, lead), Since such metal cations are known to work as micronutrients which is toxic if it’s level of concentration goes on increase. Thus this study using Atomic absorption spectroscopy (GBC Avanta Ver 1.33, Australian) leads to know the level of metallic cation is under permissible limit or not. Keywords: Antidiabetic, Extraction, Hepatoprotective, Micronutrients, Toxic. --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: 28-07-2017 Date of acceptance: 14-08-2017 --------------------------------------------------------------------------------------------------------------------------------------- I. Introduction India is known for it’s variety of medicinal herbs and plants [1], which are distributed throughout the country and known by the people living in nearby area. We find that some tribes living in remote, down trodden and neglected places do not depend on doctors for the usual medication [2, 3]. It creates a thrust of interest in researchers to know the medicinal value of plants available. Present work is done to define the level of micronutrient available in the leave extract of Aegle marmelos, Swertia chirayita, and Syzygium cumini respectively. The study of concentration of metal cation is done with the help of Atomic Absorption Spectrophotometer (GBC Avanta Ver 1.33, Australian) available at Pollution Control Board, Indore. This study is useful because medicinal plants containing metal cations like Chromium, Manganese, Iron, Nickel, Copper, and Zinc are essential nutrients but it creates harmful and toxic effects when their concentration exceeds above safe level [4]. We consider Cadmium and lead are extremely toxic even in a very less quantity [5]. The basic objective of this research work is to estimate and determine the level of concentration of different micronutrients (Chromium, Manganese, Iron, Nickel, Copper, Zinc) and toxic elements (Cadmium, lead) available in medicinal plants collected from the study sites. II. Materials and Methods 2.1 Sampling The plant leaves of Aegle marmelos, Swertia chirayita, and Syzygium cumini were collected from local market (Indore, Madhya Pradesh), were separated, cleaned with deionized water to remove impurities, shade dried at temperature not exceeding 39 0 C afterwards subjected for grinding process and then passed through 0.2 mm sieve to be obtained a uniform material in powder form, which is kept in individual plastic bags for further study. 2.2 Sample preparation and analysis 0.5 g of dried and powered sample of each plant was dissolved in 10 ml conc. HNO 3 . To increase the solubility, the sample solution was heated at 90 0 C for 2-3 hrs. After cooling sample solution transferred to a volumetric flask, make up to 50 ml and filtered through whatman filter paper No. 42. Standard solutions of the metals (Chromium, Manganese, Iron, Nickel, Copper, Zinc and Cadmium, lead) were prepared for calibration. The resulting absorbances of the metal cations were determined from the calibration graph and concentration recorded as mg/kg of metal ion using atomic absorption spectrophotometer (GBC Avanta Ver 1.33, Australian). For each plant sample two solution were prepared and results recorded in averages.